Elucidation of the Synergistic Interaction Between Bilirubin and Casein Protein: An Integrated Spectroscopy and Computational Approach.

Sudhanshu Sharma, Jyoti Vishwakarma, Jacek Czub, Subrahmanyam Sappati, Krishna Gavvala

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Article in Biomacromolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Sudhanshu SharmaDepartment of Chemistry, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana 502284, India.ORCID 0009-0001-7474-8914

Jyoti VishwakarmaDepartment of Chemistry, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana 502284, India.

Jacek CzubDepartment of Physical Chemistry, Politechnika Gdanska, 80-233 Gdańsk, Poland.ORCID 0000-0003-3639-6935

Subrahmanyam SappatiDepartment of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland.ORCID 0000-0001-9065-2253

Krishna GavvalaDepartment of Chemistry, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana 502284, India.ORCID 0000-0001-7438-2716

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Herein, we unveil the interaction between bilirubin (BIL), a liver metabolite, and a milk protein, casein (CAS), through an integrated experimental-computational approach. Encapsulation of BIL within CAS protein micelles was characterized by using UV-vis absorption, steady-state fluorescence, and circular dichroism (CD) spectroscopy. CD analysis revealed conformational modulation of BIL upon encapsulation, accompanied by Förster resonance energy transfer (FRET) from CAS's tryptophans to BIL.

Indexed as

BilirubinCaseinsCircular DichroismFluorescence Resonance Energy TransferMicellesProtein BindingSpectrometry, FluorescenceBilirubinCaseinsMicelles

Identifiers

PMID40699997

DOI10.1021/acs.biomac.5c00795

PMCPMC12344696

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