Evidence map›Paper›PMID 40699352›Full record

ArticleMikrochimica acta2025

Rapid and simultaneous detection of antibodies against three feline viruses using colloidal gold immunochromatographic assay with smartphone integration.

Jingjing Ji, Jiahao Li, Minghui Wu, Bin Wang, Yu-Nan Lu, Chuhan Zhang, Yanke Shan, Fei Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jingjing JiSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Jiahao LiSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Minghui WuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Bin WangSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Yu-Nan LuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Chuhan ZhangSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
Yanke ShanSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. shanyk26@njau.edu.cn.
Fei LiuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. feiliu24@njau.edu.cn.

Funding

Fundamental Research Funds for the Central Universities KYT2024004National Natural Science Foundation of China 22304071Start-up Research Fund for Newly Recruited PhD Researchers at Nanjing Agricultural University 804006
6 · The paper itself

Abstract

A multiplex colloidal gold immunochromatographic assay (mCGIA) was developed for the simultaneous detection of feline calicivirus (FCV), feline panleukopenia virus (FPV), and feline herpesvirus (FHV) antibodies in feline serum. The expressed and purified recombinant FCV-VP1, FPV-VP2, and FHV-gD proteins exhibited strong antigenic reactivity. The mCGIA test strip achieved rapid (< 15 min) and specific detection, showing no cross-reactivity with other feline viral antibodies. The assay achieved a low visual limit of detection (vLOD) of 1:1 × 10

Indexed as

Antibodies, ViralCalicivirus, FelineChromatography, AffinityFeline Panleukopenia VirusGold ColloidSmartphoneAnimalsCatsHerpesviridaeImmunoassayLimit of DetectionReproducibility of ResultsAntibodies, ViralGold ColloidColloidal gold immunochromatographic assayFeline virusesMultiplex antibody detectionPoint-of-care testingSmartphone-based portable reader

Identifiers

PMID40699352

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.