Evidence map›Paper›PMID 40698645›Full record

ArticleRNA biology2025

C-terminal tagging impairs AGO2 function.

Kunal M Shah, Alex F F Crozier, Anika Assaraf, Muzjda Arya, Paul Grevitt, Faraz Mardakheh, Michael J Plevin, Tyson V Sharp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Kunal M ShahCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0002-4508-9606
Alex F F CrozierCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0001-8719-5426
Anika AssarafCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Muzjda AryaCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0009-0005-5877-3326
Paul GrevittCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0001-8224-1315
Faraz MardakhehDepartment of Biochemistry, University of Oxford, Oxford, UK.ORCID 0000-0003-3896-0827
Michael J PlevinYork Structural Biology Laboratory, York Biomedical Research Institute, Department of Biology, University of York, York, UK.ORCID 0000-0003-2057-8291
Tyson V SharpCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, London, UK.ORCID 0000-0003-1861-7984

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNA-mediated gene silencing is a conserved mechanism of post-transcriptional gene regulation across metazoans. It depends on base pairing between small RNAs and mRNAs, and on protein complexes including the RNA-induced silencing complex (RISC), where Argonaute 2 (AGO2) plays a central role. A full understanding of RNA silencing requires reliable molecular tools to study AGO2 and RISC. Affinity tagging and antibody-based methods can introduce artefacts, and both the

Indexed as

Argonaute ProteinsA549 CellsAnimalsAutoantigensCRISPR-Cas SystemsDNA-Binding ProteinsHumansMicroRNAsProtein BindingRecombinant Fusion ProteinsRNA-Binding ProteinsRNA InterferenceAGO2 protein, humanArgonaute ProteinsAutoantigensDNA-Binding ProteinsMicroRNAsRecombinant Fusion ProteinsRNA-Binding ProteinsTNRC6A protein, humanAGO2ArgonauteCRISPR-Cas9HaloTagmicroRNAsprotein taggingRNAi

Identifiers

PMID40698645
PMCPMC12296108

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.