ArticleFrontiers in veterinary science2025
Non-tuberculous mycobacteria and other acid fast bacilli pathogens identification by qPCR and MALDI-ToF MS in tuberculosis-like lesions of slaughtered cattle from Ecuador.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Comparative evaluation of three commercial real-time PCR kits for direct detection ofMicrobiology spectrum · 2026Article
- Comparative evaluation of MALDI-ToF mass spectrometry and Sanger sequencing of theFrontiers in cellular and infection microbiology · 2025Article
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21 authors.
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Abstract
Introduction: Mycobacterial infections are caused by the Methods: In this study, we conducted a comprehensive inspection of 5,803 cattle carcasses destined for human consumption across 22 abattoirs in five provinces of Ecuador's coastal region, searching for lesions visually compatible with BTB (BTB-like lesions). Results: A high prevalence of 13.4% (CI 95%: 12.8-14.6) for BTB-like lesions and 3.1% (CI 95%: 2.6-3.5) for acid-fast bacilli (AFB) presence in lesions was observed. From these lesions, we isolated 48 AFB cultures, 44 of which tested positive for NTM. Strikingly, MTBC was not found in any BTB-like lesion by qPCR. Furthermore, Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-ToF MS) identified six NTM species: Discussion: Considering the potential misdiagnosis of NTM and other AFB species, BTB surveillance and control programs for MTBC should be revised to consider other opportunistic infections with similar clinical output to BTB.
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