ArticleParasites & vectors2025
Identification of tickborne pathogens in cattle and sheep ticks from Kyrgyzstan using next-generation sequencing.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Genetic uniformity and occurrence ofParasite epidemiology and control · 2026Article
- Diagnostic Insights Into Pathogen Spectrum and Mixed Microbial Detection in Critically Ill Patients With Pulmonary Infection Using Targeted Next-Generation Sequencing.Canadian respiratory journal · 2026Article
- From Panels to Pathogen Networks: The Expanding Role of Targeted Sequencing in Veterinary Medicine.Biology · 2025Review
- Molecular Profiling of Ticks and Associated Pathogens: First Report ofTransboundary and emerging diseases · 2025Article
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4 authors.
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Abstract
backgroundVarious tickborne diseases and pathogens in livestock have been reported in Kyrgyzstan; however, comprehensive molecular analyses from ticks and their tickborne pathogen diversity in the region are lacking. This study aimed to identify tick species and bacterial pathogens infesting cattle and sheep across Kyrgyzstan using amplicon-based next-generation sequencing (NGS).
methodsIn 2022, ticks were collected from livestock across seven provinces and subjected to molecular analyses. Genomic DNA was extracted from ticks for species identification based on cytochrome c oxidase I (COI) gene sequence analyses. Pathogens were screened using amplicon NGS targeting the V3-V4 region of the 16S rRNA gene, followed by confirmation using polymerase chain reaction (PCR) and Sanger sequencing.
resultsA total of 546 ticks belonging to two families, five genera, and 12 species were identified. The dominant species were Dermacentor spp. (30.2%), Hyalomma marginatum (17.2%), Hyalomma scupense (13.4%), and Haemaphysalis punctata (11.7%). Furthermore, 11.7% of ticks tested positive for bacterial pathogens, including spotted fever group Rickettsiae (8.6%), Anaplasma (2.7%), Ehrlichia (0.2%), and Bartonella (0.2%). Coxiella burnetii and Francisella tularensis were not detected.
conclusionsThis is the first nationwide study on bacterial pathogens in ticks in Kyrgyzstan and the first reports of spotted fever group Rickettsiae and Bartonella in the country. These findings improve our understanding of tickborne disease epidemiology and highlight the utility of NGS as an efficient screening method for capturing pathogen diversity in arthropod vectors.
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