Evidence map›Paper›PMID 40696096›Full record

ArticleScientific reports2025

LncRNA NRAD1 regulates the triple-negative breast cancer transcriptome by miRNA biogenesis, localization, and predominately non-ceRNA interactions.

Hannah F Cahill, Justin M Brown, Manhattan Leslie-Toogood, Jaganathan Venkatesh, Marie-Claire D Wasson, Raj Pranap Arun, Meghan E McLean, Dejan Vidovic, Paola Marcato

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hannah F CahillDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Justin M BrownDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Manhattan Leslie-ToogoodDepartment of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
Jaganathan VenkateshDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Marie-Claire D WassonDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Raj Pranap ArunDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Meghan E McLeanDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada.
Dejan VidovicDepartment of Surgery, Dalhousie University, Halifax, NS, Canada.
Paola MarcatoDepartment of Pathology, Dalhousie University, Rm 11C1, 5850 College Street, Halifax, NS, B3H 4R2, Canada. paola.marcato@dal.ca.

Funding

CIHR 162313
6 · The paper itself

Abstract

Breast cancer is a leading cause of cancer mortality in women with triple-negative breast cancer (TNBC) presenting greater treatment challenges due to its aggressive disease progression. Understanding TNBC's unique cell signaling and gene expression profiles will reveal novel therapeutic strategies. Non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), have emerged as key regulators of gene expression and potential therapeutic targets. This study focuses on a TNBC-enriched lncRNA, non-coding RNA in the aldehyde dehydrogenase 1A pathway (NRAD1, previously LINC00284), which promotes progression in multiple cancers. Our analysis reveals that NRAD1 is central to miRNA-mRNA networks in TNBC cells, mediating cancer-promoting gene expression changes. Fractionation studies showed that NRAD1 is primarily located in the nucleus and mitochondria, with some cytoplasmic presence allowing for transcript-specific competitive endogenous RNA (ceRNA) interactions with miRNAs. However, NRAD1 primarily effects miRNAs independently of ceRNA activity, instead upregulating DICER (a miRNA biogenesis protein), altering sub-cellular distribution, and reducing biogenesis of mitochondria-localized miRNA (i.e., miR-4485-3p). These findings demonstrate novel regulatory interactions between the cancer-promoting lncRNA NRAD1 and miRNAs that alter gene expression in TNBC, expanding our understanding of regulatory lncRNA-miRNA effects, TNBC biology, and highlighting future therapeutic strategies for targeting non-coding RNAs.

Indexed as

Gene Expression Regulation, NeoplasticMicroRNAsRNA, Long NoncodingTranscriptomeTriple Negative Breast NeoplasmsCell Line, TumorFemaleGene Regulatory NetworksHumansRNA, MessengerMicroRNAsRNA, Long NoncodingRNA, MessengerBreast cancerGene-expressionLINC00284Long non-coding RNAmicroRNANRAD1Triple-negative

Identifiers

PMID40696096
PMCPMC12284168

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.