Evidence map›Paper›PMID 40696062›Full record

ArticleMikrochimica acta2025

Preparation of an anti-vitamin A monoclonal antibody resistant to matrix interference and its application in quantitative immunochromatographic analysis.

Jialin Hu, Lingling Guo, Shanshan Song, Liqiang Liu, Xinxin Xu, Hua Kuang, Chuanlai Xu, Liguang Xu

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Jialin HuInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Lingling GuoInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Shanshan SongInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Liqiang LiuInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Xinxin XuInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Hua KuangInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Chuanlai XuInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
Liguang XuInternational Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China. xlg@jiangnan.edu.cn.

Funding

National Natural Science Foundation of China 32471430Scientific Research Innovation Capability Support Project for Young Faculty ZYGXQNJSKYCXNLZCXM-H11
6 · The paper itself

Abstract

Vitamin A (VA) deficiency can lead to abnormal immune system function and jeopardize health. Therefore, a simple and specific method for determining VA is needed. We analyzed the structure of hapten for the preparation of an anti-VA monoclonal antibody (mAb), assisted by computer simulation. The solution's organic solvent content was optimized during mAbs screening, taking into account its effect on antibody performance. Colloidal gold immunochromatographic assay (CGIA) strips were developed and applied to the detection of VA in infant formula, cheese, and VA dietary supplements, utilizing the prepared mAb that exhibits tolerance to 10% methanol. The visual and calculated limit of detection for infant formula, cheese, VA dietary supplements, and human plasma were 100, 50, 50 mg/kg, and 100 μg/mL and 15.06, 15.21, 27.47 mg/kg, and 20.23 μg/mL, respectively. The recoveries of CGIA strips for the detection of VA ranged from 93.67% to 102.68%. This agrees well with the liquid chromatography-tandem mass spectrometry results. Therefore, the CGIA strips could fulfill the needs for the determination of VA in real samples.

Indexed as

Antibodies, MonoclonalChromatography, AffinityVitamin AAnimalsDietary SupplementsGold ColloidHumansImmunoassayInfant FormulaLimit of DetectionAntibodies, MonoclonalGold ColloidVitamin AColloidal gold immunochromatographic assayComputer simulationMonoclonal antibody

Identifiers

PMID40696062

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.