Production of poly(3-hydroxybutyrate) by Rhodococcus qingshengii N9T-4 using the oligotrophic gene expression system.

Akihiro Otsuka, Izumi Orita, Hiroya Yurimoto, Nobuyuki Yoshida

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Akihiro OtsukaDepartment of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, 432-8561, Japan.

Izumi OritaSchool of Life Science and Technology, Institute of Science Tokyo, B-37 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.

Hiroya YurimotoDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto, 606-8502, Japan.

Nobuyuki YoshidaDepartment of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, 432-8561, Japan. yoshida.nobuyuki@shizuoka.ac.jp.ORCID https://orcid.org/0000-0002-0202-0756

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rhodococcus qingshengii N9T-4 is a super oligotroph that grows on a completely inorganic basal medium without any additional carbon and nitrogen sources. This oligotrophy may facilitate low-carbon and low-cost bioprocessing for compounds of economic significance. In this study, an operon containing three Cupriavidus necator H16 genes that are involved in polyhydroxyalkanoate (PHA) biosynthesis, phaC1AB1, was expressed in N9T-4 cells under the promoter of aldA encoding an NAD

Indexed as

HydroxybutyratesPolyestersRhodococcusBacterial ProteinsCarbonCupriavidus necatorGene ExpressionGene Expression Regulation, BacterialNitrogenOperonPolyhydroxyalkanoatesPolyhydroxybutyratesPromoter Regions, GeneticBacterial ProteinsCarbonHydroxybutyratesNitrogenpoly-beta-hydroxybutyratePolyestersPolyhydroxyalkanoatesPolyhydroxybutyrates

Identifiers

PMID40695993

DOI10.1038/s41598-025-12197-y

PMCPMC12283959

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.