Evidence map›Paper›PMID 40695979›Full record

ArticleScientific reports2025

Multi-locus study of Colletotrichum humuli from an ancient herbarium specimen and description of C. humulicola sp. nov. from Humulus scandens.

Le Dinh Thao, Hyeon-Dong Shin, Hyorim Choi, Donghun Kang, Anbazhagan Mageswari, Jae Sung Lee, Daseul Lee, In-Young Choi, Ulrike Damm, Seung-Beom Hong

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Rapid On-Site Detection ofPlants (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Le Dinh ThaoKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
Hyeon-Dong ShinDivision of Environmental Science and Ecological Engineering, Korea University, Seoul, 02841, South Korea.
Hyorim ChoiKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
Donghun KangKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
Anbazhagan MageswariKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
Jae Sung LeeKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
Daseul LeeKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea.
In-Young ChoiDepartment of Plant Medicine, Jeonbuk National University, Jeonju, 54896, South Korea.
Ulrike DammSenckenberg Museum of Natural History Görlitz, PF 300 154, 02806, Görlitz, Germany.
Seung-Beom HongKorean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, South Korea. funguy@korea.kr.

Funding

Rural Development Administration, Ministry of Science and ICT PJ01728601 and RS-2021-NR057643
6 · The paper itself

Abstract

Colletotrichum humuli was originally described from diseased leaves of Humulus lupulus collected in 1890. Due to the absence of molecular data, an isotype specimen was examined to clarify its systematic position. The nuclear ribosomal ITS2 region of this specimen was successfully amplified and sequenced using the primer ITS4 along with a newly designed primer, ITSCF1. Additional gene sequences (GAPDH, CHS-1, HIS3, and ACT) were also retrieved, marking the first successful recovery of multiple loci from an ancient Colletotrichum herbarium specimen. Multi-locus phylogenetic analyses demonstrated that C. humuli is genetically distinct from all other known species. Furthermore, comparison with isolates from H. scandens in South Korea revealed a novel species, described here as C. humulicola; together, these two species form a new species complex within the genus Colletotrichum. This study provides a straightforward protocol for obtaining DNA sequences from historical herbarium material.

Indexed as

ColletotrichumDNA, FungalMultilocus Sequence TypingPhylogenySequence Analysis, DNADNA, FungalIsotypeITSCF1Phylogenetic analysisTaxonomy

Identifiers

PMID40695979
PMCPMC12283936

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.