Evidence map›Paper›PMID 40695863›Full record

ArticleScientific reports2025

Role of Allium cepa and Allium sativum extracts in oxidative stress, sperm parameters and histological abnormalities induced by deep-frying oil in testis.

Mohamed Anwer Mohamed, Safa Hamdy Alkalash, Samah Baleegh Meawad, Mohamed Nafea Azab, Marwa A Ahmed, Ahmad Gadalla, Muhammed Abdelbaeth Hassan Elfiky, Abdel Rahman Z Abdel Rahman, Hatem Ali Ahmed Abdelmottaleb, Ayman Sabry Yassin Al Sayed and 4 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Mohamed Anwer MohamedDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Safa Hamdy AlkalashDepartment of Community Medicine and Healthcare, Faculty of Medicine, Umm Al-Qura University, Al-Qunfudha, Saudi Arabia.
Samah Baleegh MeawadDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine for Girls, Al Azhar University, Cairo, Egypt.
Mohamed Nafea AzabDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Marwa A AhmedDepartment of Food Sciences and Nutrition, College of Science, University of Bisha, P.O. Box 551, Bisha, 61922, Saudi Arabia.
Ahmad GadallaDepartment of Physiology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Muhammed Abdelbaeth Hassan ElfikyDepartment of Physiology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Abdel Rahman Z Abdel RahmanDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Hatem Ali Ahmed AbdelmottalebDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Ayman Sabry Yassin Al SayedDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.
Wafaa Yahia AlghonemyDepartment of Oral Biology, Faculty of Dentistry, Tanta University, Tanta, Egypt.
Amira OsmanDepartment of Histology and Cell Biology, Faculty of Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
Abeer A AlmowafyInternational Islamic Institute for Population Studies and Research, Al-Azhar University, Cairo, Egypt. Abeer.almowafy221@azhar.edu.eg.
Ahmed Mohamed GadAllahDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Al Azhar University, Assiut, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Palm oil is commonly used for deep-frying street food preparation. Extremely vulnerable to oxidative stress and free radicals is testicular tissue. Antioxidant supplements are advised for reducing oxidative stress, boosting spermatogenesis, and neutralizing free radicals. The study evaluated the beneficial role of Allium cepa L(AcL) and Allium sativum L (AsL) extracts in oxidative stress, histopathological, and sperm parameters abnormalities induced by deep-frying palm olein oil in rat testis. Approximately 5 kg of falafel dough tablets were daily deep-fried in oil. For three days, the frying procedure required six hours per day. The animals received daily treatment for four weeks after being split into equal groups (control, AcL, AsL, fresh palm oil (FPO), single heated palm oil (SHPO), repeated heated palm oil (RHPO), AcL + RHPO, AsL + RHPO, and AcL + AsL + RHPO). Serum testosterone level, testicular tissue of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and sperm parameters were measured. In addition, a histopathological examination of the testis was performed. Repeatedly heating oil increased considerably the MDA alongside significant depletion of GSH, SOD, and GSH-Px. Serum levels of testosterone and sperm parameters were decreased significantly with testicular damage. Considerable improvements in oxidative stress, sperm parameters, and testicular histology were observed with concomitant administration of AcL and AsL. SHPO showed no significant changes. In the testes of rats subjected to oxidative stress caused by RHPO, extracts of AcL and AsL exhibit strong antioxidant protection. The study concluded that AcL and AsL extracts have potent antioxidant protection in the testes of rats exposed to RHPO-induced oxidative stress.

Indexed as

GarlicOnionsOxidative StressPlant ExtractsSpermatozoaTestisAnimalsAntioxidantsGlutathioneMaleMalondialdehydePalm OilPlant OilsRatsRats, WistarSuperoxide DismutaseAntioxidantsGlutathioneMalondialdehydePalm OilPlant ExtractsPlant OilsSuperoxide DismutaseTestosteroneAcLAsLOxidative stressPalm oilTestes

Identifiers

PMID40695863
PMCPMC12284171

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.