Evidence map›Paper›PMID 40695104›Full record

ReviewBest practice & research. Clinical obstetrics & gynaecology2025

Is the time right for transplanting immature testicular tissue or cells to restore male fertility? Expert perspectives on clinical implementation of autotransplantation of cryopreserved testicular tissue or cells for fertility restoration.

Myriam Safrai, Ellen Goossens, Rod T Mitchell, Kyle E Orwig, Callista L Mulder, Ans M M van Pelt, Debra A Gook, Aurélie Feraille, Emily Delgouffe, Jill P Ginsberg and 12 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Best practice & research. Clinical obstetrics & gynaecology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Myriam SafraiIVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center (Tel Hashomer), Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Ellen GoossensBiology of the Testis (BITE) Laboratory, Research Group Genetics, Reproduction and Development (GRAD), Vrije Universiteit Brussel, (VUB), 1090, Brussels, Belgium.
Rod T MitchellCentre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, Edinburgh, UK; Royal Hospital for Children and Young People, Edinburgh, UK. Electronic address: rod.mitchell@ed.ac.uk.
Kyle E OrwigDepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Callista L MulderReproductive Biology Laboratory, Center for Reproductive Medicine, Amsterdam UMC Location University of Amsterdam, Amsterdam, the Netherlands; Amsterdam Reproduction and Development Research Institute, Amsterdam, the Netherlands.
Ans M M van PeltReproductive Biology Laboratory, Center for Reproductive Medicine, Amsterdam UMC Location University of Amsterdam, Amsterdam, the Netherlands; Amsterdam Reproduction and Development Research Institute, Amsterdam, the Netherlands.
Debra A GookReproductive Services, The Royal Women's Hospital, Parkville, VIC, Australia; Department of Obstetrics and Gynaecology, Royal Women's Hospital, University of Melbourne, Parkville, VIC, Australia.
Aurélie FerailleNorDIC, Team "Adrenal and Gonadal Pathophysiology, " Biology of Reproduction-CECOS Laboratory, Rouen University Hospital, Rouen Normandy University, Rouen, France.
Emily DelgouffeBiology of the Testis (BITE) Laboratory, Research Group Genetics, Reproduction and Development (GRAD), Vrije Universiteit Brussel, (VUB), 1090, Brussels, Belgium.
Jill P GinsbergDivision of Oncology, Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Jan-Bernd StukenborgNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, and Karolinska University Hospital, Solna, Sweden; NORDFERTIL Research Lab Uppsala, Department of Organismal Biology, Uppsala University, Uppsala, Sweden.
Kathleen DuffinCentre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, Edinburgh, UK; Royal Hospital for Children and Young People, Edinburgh, UK.
Kirsi JahnukainenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, and Karolinska University Hospital, Solna, Sweden; Division of Haematology-Oncology and Stem Cell Transplantation, New Children's Hospital, Pediatric Research Center, Department of Pediatrics, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Claus Yding AndersenThe Fertility Clinic and the Urological Department Herlev University Hospital of Copenhagen & Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Marianne D van de WeteringPrincess Maxima Center for Pediatric Oncology, Utrecht, the Netherlands.
Michael P RimmerCentre for Reproductive Health, Institute of Regeneration and Repair, University of Edinburgh, Edinburgh, UK.
Virginie Barraud-LangeDepartment of Reproductive Biology CECOS, Public Assistance-Paris Hospital (AP-HP) Paris Centre University Hospital (HUPC), Cochin Hospital, Paris, France; AYA Unit, Fertility Preservation Consultation, Haematology Department, Public Assistance-Paris Hospital (AP-HP), Paris North University Hospital (HUPN), Nord, University of Paris Cité, Saint-Louis Hospital, Paris, France.
Nina NeuhausCentre of Reproductive Medicine and Andrology, University of Münster, Münster, Germany.
Sheila LaneNuffield Department of Women's & Reproductive Health, University of Oxford, England, UK.
Hooman Sadri-ArdekaniInstitute of Regenerative Medicine and Department of Urology, Atrium Health Wake Forest Baptist, Winston-Salem, NC, 27157, USA.
Nathalie RivesNorDIC, Team "Adrenal and Gonadal Pathophysiology, " Biology of Reproduction-CECOS Laboratory, Rouen University Hospital, Rouen Normandy University, Rouen, France.
Christine WynsDepartment of Gynecology and Andrology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.

Funding

Next Generation Therapies for Fertility Preservation in Male Cancer PatientsR01HD100197 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI MEISTRICH, MARVIN L., ORWIG, KYLE EDWIN · 2020 to 2024
$4.0M
NICHD NIH HHS R01 HD100197
6 · The paper itself

Abstract

Transplantations of cryopreserved immature testicular tissue (ITT) or spermatogonial stem cells (SSCs) represent promising approaches to restore fertility in patients with testicular tissue or cell suspension cryopreserved for fertility preservation. With over 3000 testicular samples cryopreserved globally, clinically viable and standardized protocols restoring fertility using cryopreserved testicular tissue/cells are urgently needed. Decades of research demonstrate the feasibility of immature testicular tissue transplantation (ITTT) or spermatogonial stem cell transplantation (SSCT) in animal models, including non-human primates. However, significant challenges remain in translating these options to human clinical practice. Critical factors include rigorous patient selection, robust pre-transplant evaluations to mitigate risks of malignant cell reintroduction, and the optimization of transplantation timing to support spermatogenesis. Developing comprehensive follow-up protocols and international data-sharing frameworks is essential to optimize outcomes. While offering the potential for genetic parenthood and enhanced quality of life for cancer survivors, these techniques require further refinement to ensure safety, efficacy, and realistic expectations. This paper outlines the framework for advancing the clinical translation of autotransplantation of testicular tissue/cells through collaboration and innovation.

Indexed as

Adult Germline Stem CellsCryopreservationFertility PreservationInfertility, MaleTestisAnimalsHumansMaleTransplantation, AutologousFertility preservationImmature testicular tissuePre-pubertal boysSpermatogonial stem cell transplantationTesticular tissue cryopreservationTesticular tissue transplantation

Identifiers

PMID40695104
PMCPMC13229609

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.