ArticleJournal of immunology (Baltimore, Md. : 1950)2025
Refined cell transfer model reveals roles for Ascl2 and Cxcr3 in splenic localization of mouse NK cells during virus infection.
Laura M Canaday, Andrew Cox, H Alex Feldman, Harsha Seelamneni, Ayad Ali, Jasmine A Tuazon, Lorena Botero Calderon, Sierra N Bennett, Allison Yan, Megan Wilson and 2 more
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In one paragraphArticle in Journal of immunology (Baltimore, Md. : 1950), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
12 authors.
Laura M CanadayImmunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States.
Andrew CoxDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.ORCID 0000-0003-3485-8892 H Alex FeldmanImmunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States.ORCID 0000-0002-3281-2535 Harsha SeelamneniDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.
Ayad AliImmunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States.
Jasmine A TuazonDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.ORCID 0000-0001-9896-9714 Lorena Botero CalderonDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.
Sierra N BennettDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.ORCID 0009-0006-9611-4046 Allison YanDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.
Megan WilsonDivision of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.ORCID 0009-0009-8346-257X Vijayakumar VeluDivision of Microbiology and Immunology, Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, United States.ORCID 0000-0003-4238-1924 Stephen N WaggonerImmunology Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States.ORCID 0000-0001-9658-2874 Funding
Leadership Group for the Infectious Diseases Clinical Research Consortium (IDCRCLG) - Momi-Vax DMID #21-0004 {Supplement #6}UM1AI148684 · NIAID · EMORY UNIVERSITY · PI DAVID S STEPHENS · 2020 to 2026
$77.2MPediatric Scientist Development Program (PSDP) [K12]K12HD000850 · NICHD · YALE UNIVERSITY · PI Sallie R. Permar · 1987 to 2026
$44.1MStem Cell/Organoid and Genome Editing CoreP30DK078392 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI LEE ARMISTEAD DENSON · 2007 to 2026
$24.4MCincinnati Children's Hospital Medical Center Vaccine and Treatment Evaluation Units (UM1 Clinical Trial Required) - Moderna Pediatric StudyUM1AI148372 · NIAID · CINCINNATI CHILDRENS HOSP MED CTR · PI Robert Wilson Frenck · 2020 to 2026
$20.5MTissue Repository CoreP30AR070549 · NIAMS · CINCINNATI CHILDRENS HOSP MED CTR · PI Leah Claire Kottyan · 2016 to 2026
$7.7MMedical Scientist Training ProgramT32GM063483 · NIGMS · UNIVERSITY OF CINCINNATI · PI KHURANA HERSHEY, GURJIT K. · 2002 to 2022
$6.7MTargeting natural killer cells to enhance HIV vaccine responsesR01AI148080 · NIAID · CINCINNATI CHILDRENS HOSP MED CTR · PI WAGGONER, STEPHEN N. · 2019 to 2023
$3.3MBlocking granzyme-mediated immune suppression to enhance HIV vaccine efficacyR01AI176519 · NIAID · CINCINNATI CHILDRENS HOSP MED CTR · PI Vijayakumar Velu, Stephen N. Waggoner · 2023 to 2026
$2.8MTranscription Factor Genetics in LupusR01AR073228 · NIAMS · CINCINNATI CHILDRENS HOSP MED CTR · PI KOTTYAN, LEAH CLAIRE, WAGGONER, STEPHEN N. · 2019 to 2023
$2.2MVaccinology Training ProgramT32AI165396 · NIAID · CINCINNATI CHILDRENS HOSP MED CTR · PI Robert Wilson Frenck, PAUL W. SPEARMAN · 2022 to 2026
$1.4MPathogenesis and therapeutic targeting of immune disordersT32AI118697 · NIAID · CINCINNATI CHILDRENS HOSP MED CTR · PI HILDEMAN, DAVID A · 2016 to 2020
$683kAlbert J Ryan FoundationCincinnati Children's Hospital Flow Cytometry Core AR070549Cincinnati Children's Research FoundationCincinnati Research In Residency ProgramDK078392Infectious Disease Clinical Research Consortium Pilot Award UM1AI148684NIAID NIH HHS R01 AI148080NIAID NIH HHS R01 AI176519NIAID NIH HHS T32 AI118697NIAID NIH HHS T32 AI165396NIAID NIH HHS UM1 AI148372NIAID NIH HHS UM1 AI148684NIAMS NIH HHS P30 AR070549NIAMS NIH HHS R01 AR073228NICHD NIH HHS K12 HD000850NIDDK NIH HHS P30 DK078392NIGMS NIH HHS T32 GM063483NIH HHS K12-HD000850NIH HHS R01AI148080NIH HHS R01AI176519NIH HHS R01AR073228NIH HHS T32AI118697NIH HHS T32AI165396NIH HHS T32GM063483Pediatric Scientist Development Program supported
6 · The paper itselfAbstract
Cell transfer experiments complement the rigorous investigation of antiviral and antitumor functions of natural killer (NK) cells. Success in these endeavors is enhanced by expansion of small numbers of input NK cells driven by viral antigens or homeostatic proliferation in immunodeficient hosts. In contrast, analysis of other NK-cell functions, including immunoregulation, are non-proliferative and require an intact immune system in recipient mice. We reveal poor persistence of conventional congenic (CD45.1) BoyJ NK cells following adoptive transfer in comparison to CRISPR-generated CD45.1+ (JAXBoy) NK cells. Reciprocal transfers between C57BL/6 and JAXBoy mice substantially improve seeding and maintenance of donor NK cells. Using this system, we confirm that CXCR3 re-positions NK cells in the white pulp of the spleen after infection, which is vital for immunoregulation. Moreover, we discovered that the transcription factor ASCL2 is required for recruitment of NK cells into the spleen and white pulp. These results provide improved tools and novel insights into NK cell biology.
Indexed as
Basic Helix-Loop-Helix ProteinsKiller Cells, NaturalReceptors, CXCR3SpleenVirus DiseasesAdoptive TransferAnimalsMiceMice, Inbred C57BLBasic Helix-Loop-Helix ProteinsCxcr3 protein, mouseReceptors, CXCR3chemotaxisimmune suppressioninnate lymphoid cellmigrationvaccine
Identifiers
PMID40694683
PMCPMC12313101
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