Article3 Biotech2025
A reverse transcription-recombinase polymerase amplification (RT-RPA) assay for rapid and sensitive detection of tobacco streak virus causing sunflower necrosis disease.
Article in 3 Biotech, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Development of RT-RPA assays for rapid detection of mango-infecting viruses using high-throughput sequencing-derived genomic information.BMC plant biology · 2026Article
- Molecular Characterization of an Isolate of Tobacco Streak Virus Naturally InfectingPlants (Basel, Switzerland) · 2026Article
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13 authors.
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Abstract
Sunflower necrosis disease (SND), caused by tobacco streak virus (TSV), is an economically important disease of sunflower. Rapid and sensitive detection of TSV is crucial for the management of SND. In the present study, we developed an isothermal amplification assay based on the reverse transcription-recombinase polymerase amplification (RT-RPA) technique for the first time for rapid and sensitive detection of TSV using crude leaf sap of infected plants, obtained by crushing the samples in Tris extraction buffer (pH 7.5, 1 M) as a template. For reliable detection of TSV in crude leaf sap using the developed assay, the reaction mixture was incubated at 38 °C for 30 min. The developed assay was specific to the TSV and detected the virus up to 10 Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-025-04419-x.
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