ArticleFrontiers in neuroscience2025
Sensory-spinal neuron co-culture platform enables analysis of sensory-driven spinal activation.
Article in Frontiers in neuroscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- In Vitro Reconstruction of Axonal Heat Sensing with a Photothermal Nerve-on-a-Chip.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
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4 authors.
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Abstract
Background: Pain plays a crucial role in selecting behaviors essential for survival. Nociceptive stimuli are converted into neuronal signals by dorsal root ganglion (DRG) neurons and transmitted via the spinal cord to the brain, where pain is perceived. Chronic pain, characterized by prolonged nociceptive signaling, significantly reduces the quality of life. Specifically, nociplastic pain arises due to heightened spinal neuronal activity. However, the mechanisms underlying this persistent increase remain unclear, impeding the development of effective treatments. Therefore, the present study aimed to develop an experimental platform to investigate how sensory neuron signals increase spinal neuronal activity. Methods: We developed a specialized microstructure enabling a separate culture of DRG and spinal neurons connected functionally by axons extending through microtunnels. Results: Immunofluorescence staining confirmed precise spatial separation and robust neuronal network formation. Microstructures were integrated with high-density microelectrode arrays to facilitate electrophysiological recordings during co-culture. Optogenetic stimulation of DRG neurons significantly activate the spinal neurons, which are not active spontaneously, and increase synchronous activity by 11.8-fold in the spinal neuronal network. Notably, elevated spinal neuron activity persisted for at least 20 min after stimulation ceased, indicating a prolonged neuronal response. Conclusion: This novel co-culture system provides a powerful tool for elucidating the pathogenic mechanisms underlying chronic pain, potentially guiding future therapeutic strategies.
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