Evidence map›Paper›PMID 40692554›Full record

ArticleJournal of nutritional science2025

Legume lectin phytohemagglutinin reduces transepithelial electrical resistance by counteracting the chaperone function of heat shock protein-70.

Karol Dokladny, Prashanth Setty, Pope L Moseley, Henry C Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nutritional science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Karol DokladnyDepartment of Medicine, University of New Mexico, Albuquerque, NM, USA.ORCID https://orcid.org/0000-0003-4912-9051
Prashanth SettySiemens Healthcare Molecular Diagnostics, Berkeley, CA, USA.
Pope L MoseleyNovo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Henry C LinSection of Gastroenterology, New Mexico Veteran Affairs Health Care System, Albuquerque, NM, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Legume lectins represent a broad class of environmental toxicants that bind to cell surface glycoproteins. Raw red kidney beans (RRKB), a widely consumed common source of dietary protein, are rich in the lectin phytohemagglutinin (PHA). Consumption of improperly cooked (which may require overnight presoaking and boiling at least at 100°C for 45 min) red kidney beans causes severe gastrointestinal symptoms. Since the relationship between lectin toxicity and the cellular chaperone machinery remains unknown, the study aimed to determine the effects of heat-denatured PHA on epithelial barrier function and heat shock protein 70 (HSP70) expression and its function as a molecular chaperone in PHA-treated Caco-2 cells and animals. Twelve male Sprague-Dawley rats were randomised to an

Indexed as

FabaceaeHSP70 Heat-Shock ProteinsIntestinal MucosaPhytohemagglutininsAnimalsCaco-2 CellsElectric ImpedanceHumansIntestine, SmallMaleMolecular ChaperonesRatsRats, Sprague-DawleyHSP70 Heat-Shock ProteinsMolecular ChaperonesPhytohemagglutininsAd70,heat shock protein 70 overexpressing adenovirusAdTrack,control adenovirusCaco-2,cancer coli-2Cat.,cataloguecDNA,complementary deoxyribonucleic acidCHIP,carboxy terminus of heat shock protein 70-interacting proteinCO2,carbon dioxideCT,cycle thresholdDAPI,4’,6-diamidino-2-phenyindoleDNA,deoxyribonucleic acidEDTA,ethylenediaminetetraacetic acidELISA,enzyme-linked immunosorbent assayFITC,fluorescein-5-isothiocyanateHOP,heat shock protein 70-heat shock protein 90 organising proteinHRP,horseradish peroxidaseHSC,heat shock cognateHSF1HSF,heat shock factorHSP70HSP,heat shock proteinIG,immunoglobulinsIL,interleukinkDa,kilodaltonsKFERQ,lysine-phenylalanine-glutamic acid-arginine-glutamineLAMP,lysosomal-associated membrane proteinLectinsNaCl,sodium chlorideNaF,sodium fluoridePBS,phosphate-buffered salinePCR,polymerase chain reactionpGL3,luciferase reporter vectorPHA,phytohemagglutininPhytohemagglutinin (PHA)PMSF,phenylmethylsulfonyl fluorideProtein foldingQPCR,quantitative polymerase chain reactionRAW,reticuloendothelial anti-cancer agentRNA,ribonucleic acidRPM,revolutions per minuteRRKB,raw red kidney beansrRNA,ribosomal ribonucleic acidSDS-PAGE,sodium dodecyl sulfate polyacrylamide gel electrophoresisSD,standard deviationTBS,tris-buffered salineTEER,transepithelial electrical resistanceTNF,tumour necrosis factorTris-HCl,tris(hydroxymethyl)aminomethane hydrochlorideTween 20,polyoxyethylene (20) sorbitan monolaurateZO-1,zonula occludens-1

Identifiers

PMID40692554
PMCPMC12278180

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.