Evidence map›Paper›PMID 40691858›Full record

ArticleVeterinary research2025

Heterologous signal peptide grafting enhances the immune efficacy of Salmonella vectors delivering hemagglutinin against H7N9 avian influenza virus.

Wangyangji Sun, Rui Zhu, Yu-An Li, Zewei Li, Yuanzhao Du, Shifeng Wang, Huoying Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wangyangji SunCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Rui ZhuCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Yu-An LiCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Zewei LiCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Yuanzhao DuYebio Bioengineering Co., Ltd of Qingdao, Qingdao, 266114, China.
Shifeng WangDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611-0880, USA.
Huoying ShiCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China. hyshi@yzu.edu.cn.ORCID http://orcid.org/0000-0003-4128-329X

Funding

National Key R&D Program of China 2023YFE0123500The National Natural Science Foundation of China 31672516The National Natural Science Foundation of China 32302823The Natural Science Foundation of Jiangsu Province BK20230576The Postgraduate Research & Practice Innovation Program of Jiangsu Province No. KYCX21-3266The Postgraduate Research & Practice Innovation Program of Jiangsu Province Yangzhou UniversityThe State Key Laboratory of Genetically Engineered Veterinary Vaccines No. AGVSKL-ZD-202004
6 · The paper itself

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) vectors, which induce broad cellular and humoral immune responses, are excellent candidates for delivering foreign antigens. However, S. Typhimurium strains display limitations, including low levels of antigen protein expression when delivering viral antigens. In this study, we found that replacing the hemagglutinin (HA) precursor sequence of H9N2 AIV (avian influenza virus) with that from H7N9 AIV significantly improved HA protein expression. Building on this, we combined the H9N2 HA leader sequence with a tissue plasminogen activator (tPA) signal peptide and delayed lysis Salmonella mRNA interferase regulation vector (SIRV) system previously developed by our team. This novel approach markedly enhanced the expression of viral antigens delivered by Salmonella vectors. Our results demonstrate that both the H9N2 HA leader sequence and the tissue plasminogen activator (tPA) signal peptide significantly increased H7N9 AIV HA protein expression and substantially improved the protective efficacy of the attenuated S. Typhimurium vector delivering the H7N9 HA protein vaccine against H7N9 AIV challenge. These findings offer valuable insights for developing more effective attenuated Salmonella-based recombinant H7N9 AIV vaccines and provide a valuable reference for vaccine strategies against other infectious diseases.

Indexed as

Hemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H7N9 SubtypeInfluenza in BirdsInfluenza VaccinesProtein Sorting SignalsSalmonella typhimuriumAnimalsChickensGenetic VectorsHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesProtein Sorting SignalsH7N9 avian influenza virusInfluenza virusSalmonella vectortissue plasminogen activator signal sequence

Identifiers

PMID40691858
PMCPMC12282016

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.