ArticleBMC biotechnology2025
Biogenic silver nanoparticles synthesized from Pseudomonas fluorescens-mediated olive cake waste: antimicrobial, larvicidal activity against Culex pipiens and cytotoxicity assessment.
Article in BMC biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Box-Behnken-Optimized Bacterial Nanocellulose-Silver and Zinc Oxide Nanocomposites: Bactericidal Activity, Antioxidant Capacity, Cytotoxicity, and In vitro Wound Healing Assessment.Applied biochemistry and biotechnology · 2026Article
- Exopolysaccharide-mediated silver oxide nanoparticles from Bacillus cereus GV7: green synthesis, characterization, and antimicrobial, antibiofilm, antioxidant, and anticancer activities.Archives of microbiology · 2026Article
- Phyto-capped CuO Nanoparticles with Enhanced Antimicrobial and Anticancer Efficacy Against MCF-7 Cell Line: A Green Nanotherapeutic Approach.Applied biochemistry and biotechnology · 2026Article
- Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity.Bioresources and bioprocessing · 2026Article
- Berberine-ZnO loaded chitosan nanoparticles inhibits biofilm formation in Pseudomonas aeruginosa PAO1 through targeting extracellular polymeric substances.BMC biotechnology · 2026Article
- Antibiotic-phytochemical combinations against Enterococcus faecalis: a therapeutic strategy optimized using response surface methodology.Antonie van Leeuwenhoek · 2026Article
- Harnessing copper oxide nanoparticles for advanced photocatalytic, antimicrobial, and larvicidal applications.Scientific reports · 2025Article
- Green synthesis of silver nanoparticles from Bacillus subtilis-mediated feather hydrolysate: antimicrobial, larvicidal against culex pipiens, and anticancer activities.Bioresources and bioprocessing · 2025Article
- Green synthesis of silver iodide nanoparticles from Kluyveromyces marxianus M59 beta-glucan: characterization and anticancer activity.BMC biotechnology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study presents an eco-friendly approach for synthesizing silver nanoparticles (AgNPs) using olive cake hydrolysate (OCH), produced through microbial fermentation of olive cake waste by Pseudomonas fluorescens. The OCH was analyzed by gas chromatography-mass spectrometry (GC-MS), revealing the biotransformation of olive cake components into bioactive compounds, including 24-norursa-3,12-diene, methyl esters of 9,12-octadecadienoic acid and 9-octadecenoic acid, and α-sitosterol. The biosynthesized olive cake hydrolysate-silver nanoparticles (OCH-AgNPs) were characterized using ultraviolet-visible (UV-Vis) spectroscopy to confirm surface plasmon resonance at 420 nm; Fourier-transform infrared (FTIR) spectroscopy to identify the involvement of hydroxyl and carbonyl functional groups; X-ray diffraction (XRD) analysis to verify the crystalline structure, revealing prominent (111) lattice planes of face-centered cubic (fcc) silver; transmission electron microscopy (TEM) to assess morphology and particle size, showing spherical nanoparticles with an average diameter of 19.6 ± 6.1 nm; dynamic light scattering (DLS) to measure hydrodynamic diameter, yielding a size of 109.8 nm; and zeta potential analysis to determine surface charge, which indicated high colloidal stability with a zeta potential of - 47.0 mV. OCH-AgNPs exhibited superior antimicrobial activity compared to OCH alone, with low MIC values against P. aeruginosa, Candida albicans, Aspergillus brasiliensis, and Staphylococcus aureus MRSA. Larvicidal activity, optimized via Box-Behnken design, showed 98.86% mortality of Culex pipiens at 1.0 µg/mL (LC₅₀ = 0.40 µg/mL), significantly outperforming OCH (LC₅₀ = 57.22 µg/mL). Histopathological and biochemical analyses of treated larvae revealed structural damage, decreased protein and carbohydrate content, and inhibition of acetylcholinesterase. Cytotoxicity assays on human skin fibroblasts confirmed low toxicity (IC₅₀ >200 µg/mL). Molecular docking identified α-sitosterol as a key bioactive component. These findings underscore the potential of OCH-AgNPs as a sustainable and multifunctional biocontrol agent for microbial and vector management.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.