ArticleScientific reports2025
Exploring the impact of forage-to-concentrate ratios on the ruminal bacteriome in vitro focusing on ciliate-associated bacteria.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Lactobacillus Modulates the Rumen Microbiota and Transcriptome to Enhance Nutrient Digestion in Yaks Fed High-Concentrate Diets During the Cold Season.Animals : an open access journal from MDPI · 2026Article
- Effects of Oral Administration of Goat Rumen-Derived Bacterial Isolates on In Vitro and In Vivo Rumen Fermentation Characteristics.Animals : an open access journal from MDPI · 2026Article
- Effects of Dietary Concentrate-to-Roughage Ratio on Rumen Microbiota, Functional Profiles, and Fermentation Characteristics in Yak.Microorganisms · 2026Article
- Dietary Supplementation of Concentrate Improves Lactation Performance and Immune Function in Grazing Lactating Yaks Through Changes in Rumen Microbial Population and Metabolites.Microorganisms · 2026Article
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Authors and funding
3 authors.
Funding
Abstract
Ruminal ciliates are linked to methane production and nitrogen utilization efficiency in ruminants due to their association with other ruminal microorganisms. However, research on the specific interplay between ruminal bacteria and ciliates is still limited, particularly in different dietary conditions. This study examines the effect of the forage-to-concentrate (F:C) ratio on the ruminal bacteriome in vitro, focusing on bacteria associated with Isotricha spp. and small entodinia. The rumen fluid used as the inoculum for this experiment was collected from two cannulated Hanwoo cows. Dietary treatments included high-forage (HF, F:C of 7:3), high-concentrate (HC, F:C of 3:7), and control (CON, F:C of 5:5). After 24-hour incubation, fractions for entodinia-associated bacteria (EAB), Isotricha-associated bacteria (IAB), and total bacteria (TB) were collected for bacteriome analysis using QIIME2 with full-length 16S rRNA gene sequences on the PacBio system. All fermentation parameters, except for NH
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