Evidence map›Paper›PMID 40691465›Full record

ArticleScientific data2025

Transcriptome profiling and co-expression network analysis of 96 Haematococcus pluvialis samples.

Bin Hu, Kai Liu, Feichao Du, Hailiang Xing, Xiaoyuan Su, Tao Hu, Feng Chen, Yujie Qin, Zhaozhen Dong, Xue Sun and 4 more

Abstract readDataset
In one paragraph

Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Exploring the Potential ofMicroorganisms · 2025
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Bin HuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Kai LiuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Feichao DuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Hailiang XingKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Xiaoyuan SuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Tao HuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Feng ChenKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Yujie QinKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Zhaozhen DongKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Xue SunKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Liuquan ZhangKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.
Jagroop PandhalDepartment of Chemical and Biological Engineering, University of Sheffield, Sheffield, United Kingdom.
Nianjun XuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China. xunianjun@nbu.edu.cn.
Chaoyang HuKey Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China. huchaoyang@nbu.edu.cn.

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32373152
6 · The paper itself

Abstract

Haematococcus pluvialis is a microalga known for producing the red carotenoid, astaxanthin. Key research areas to improve productivity include optimizing vegetative biomass, enhancing astaxanthin content, and controlling secondary cell wall formation. In this study, RNA-seq was conducted on 96 H. pluvialis samples under various treatments and time points, generating 96.7 GB of high-quality data (2,080,511,173 clean reads, quality score > 30). Gene expression was quantified as transcripts per million (TPM), and genes with similar expression patterns were clustered. A co-expression network, constructed with a soft threshold of β = 7 (R² > 0.9), identified 39 co-expression modules, each averaging 732 genes. Additionally, the re-annotation process provided functional descriptions for 8,598 previously uncharacterized genes. Gene functional classification analysis revealed their involvement in metabolic pathways related to genetic information processing and primary/secondary metabolite metabolism. This dataset provides a valuable resource for exploring the molecular mechanisms involved in critical processes such as growth regulation, astaxanthin accumulation, and secondary cell wall synthesis in H. pluvialis.

Indexed as

ChlorophyceaeChlorophytaGene Regulatory NetworksTranscriptomeGene Expression ProfilingRNA-SeqXanthophyllsastaxanthineXanthophylls

Identifiers

PMID40691465
PMCPMC12280055

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.