Evidence map›Paper›PMID 40691076›Full record

ArticleExperimental animals2026

Suppression of USP2 in mouse skeletal muscle: a model of oxidative stress in muscle tissue.

Masaki Fujimoto, Tomohito Iwasaki, Marina Hosotani Saito, Naoki Takahashi, Mayuko Hashimoto, Eiki Takahashi, Hiroshi Kitamura

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental animals, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Masaki FujimotoLaboratory of Disease Models, School of Veterinary Medicines, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
Tomohito IwasakiDepartment of Food Science and Human Wellness, College of Agriculture, Food and Environment Science, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
Marina Hosotani SaitoDepartment of Otolaryngology, University of Texas Medical Branch, 400 Harborside Dr Unit 123, Galveston, Texas 77555-0521, USA.
Naoki TakahashiLaboratory of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
Mayuko HashimotoLaboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Kita 3-11-1, Nishikiori, Tondabayashi, Osaka 584-8540, Japan.
Eiki TakahashiDepartment of Biomedicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Hiroshi KitamuraDepartment of Laboratory Animal Medicine, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Emerging evidence indicates that oxidative stress in skeletal muscle is a prerequisite for sarcopenia in diabetic patients. In this study, we show that ubiquitin-specific protease (USP) 2 mitigates the accumulation of reactive oxygen species (ROS) in mature muscle cells. Treatment with ML364, a canonical USP2 inhibitor, robustly increased mitochondrial ROS in mouse C2C12 myotubes and caused an accompanying increase in the glutathione disulfide (GSSG)/glutathione (GSH) ratio. ML364 also caused mitochondrial damage in C2C12 myotubes, resulting in a reduction in intracellular adenosine triphosphate levels. Correspondingly, under diabetic condition, the muscle-specific Usp2-knockout (msUsp2KO) C57BL/6N mice exhibited a significantly higher lipid peroxide level and GSSG/GSH ratio in skeletal muscle than the control mice. The msUsp2KO mice also exhibited augmented insulin resistance and glucose intolerance, but showed no obvious deterioration in muscle weight or histology relative to the control mice. However, damaged mitochondria in the soleus muscle were more frequently observed in msUsp2KO mice than in the control mice. Together, these data suggest that USP2 mitigates ROS accumulation and subsequent mitochondrial damage in muscle cells in mice.

Indexed as

Muscle, SkeletalOxidative StressUbiquitin ThiolesteraseAnimalsInsulin ResistanceMaleMiceMice, Inbred C57BLMice, KnockoutMitochondriaMuscle Fibers, SkeletalReactive Oxygen SpeciesReactive Oxygen SpeciesUbiquitin ThiolesteraseUsp2 protein, mousediabetesoxidative stressskeletal muscleubiquitin-specific protease (USP) 2

Identifiers

PMID40691076
PMCPMC12826574

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.