Evidence map›Paper›PMID 40690723›Full record

ArticleTissue engineering. Part C, Methods2025

Optimization of Cytometry by Time-of-Flight Staining for Peripheral Blood and Bone Marrow Samples.

Yosuke Susuki, Issei Shinohara, Masatoshi Murayama, Mayu Morita, Chao Ma, Alexa K Pius, Qi Gao, Simon Kwoon-Ho Chow, Stuart B Goodman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part C, Methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yosuke SusukiDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Issei ShinoharaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Masatoshi MurayamaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Mayu MoritaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Chao MaDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Alexa K PiusDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Qi GaoDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.
Simon Kwoon-Ho ChowDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.ORCID 0000-0003-1742-980X
Stuart B GoodmanDepartment of Orthopaedic Surgery, Stanford University School of Medicine, Stanford, California, USA.

Funding

Autologous Bone Marrow Aspirate Concentrate for the Treatment of Osteonecrosis of the Femoral HeadU01AR080993 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI STUART B GOODMAN, Lynne C. Jones · 2023 to 2026
$2.4M
NIAMS NIH HHS U01 AR080993
6 · The paper itself

Abstract

Cytometry by time-of-flight (CyTOF) enables comprehensive immune profiling for translational research. However, challenges such as signal variability, nonspecific binding, and antibody incompatibility can compromise data quality. This study presents an optimized CyTOF staining protocol for human peripheral blood mononuclear cells and bone marrow aspiration concentrate samples, addressing these challenges by refining antibody conjugation with polymer X8, saponin use, and fixation protocols. Preliminary data indicate improved staining for key markers (CD14, CD16, and CD19), enhancing signal consistency and clarity. These findings advance the utility of CyTOF in orthopaedic research and immune profiling for diseases such as osteonecrosis of the femoral head.

Indexed as

Bone MarrowBone Marrow CellsFlow CytometryLeukocytes, MononuclearStaining and LabelingHumansbone marrow aspiration concentrateCyTOFosteonecrosis of the femoral headperipheral blood mononuclear cells

Identifiers

PMID40690723
PMCPMC13633060

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.