ArticleApplied microbiology and biotechnology2025
Knockout of glycerol metabolic pathways enables efficient mycolicibacterial phytosterol conversion using glycerol as cosovlent.
Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Direct Conversion of Phytosterol to Testosterone via an Integrated Microbial-Enzymatic Strategy.Chemistry & biodiversity · 2026Article
- Potential of sustainable, ecofriendly sterol derivatives as additives for water and oil repellency.Scientific reports · 2026Article
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7 authors.
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Abstract
Mycobacterial phytosterol conversion naturally occurs within a vegetable oil environment, a process that has been widely adopted in water‒oil two-phase fermentation to produce active pharmaceutical intermediates of steroids. The use of hydroxypropyl-β-CD (HP-β-CD) as a potential replacement for vegetable oils has been explored. However, both approaches encounter challenges, particularly the need for a high concentration of either vegetable oil or HP-β-CD in the fermentation medium, which significantly affects the efficiency of mycobacterial phytosterol conversion as well as the recovery of the resulting products. In this study, we demonstrated that the knockout of glycerol metabolic pathways enables the utilization of glycerol as cosovlent for efficient phytosterol conversion in Mycolicibacterium neoaurum HGMS6, an industrial strain known for its production of 4-androstene-3,17-dione (4-AD). Our bioinformatic analysis revealed two parallel glycerol metabolic pathways in the HGMS6 strain. These pathways are independently initiated by glycerol kinase (GLPK) and glycerol dehydrogenase (ADHC). To investigate their roles, we conducted gene knockout experiments in which both the glycerol kinase gene (glpk) and the glycerol dehydrogenase gene (adhc) were targeted in HGMS6, resulting in the creation of a glycerol metabolism-deficient mutant, denoted HGMS6
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