Evidence map›Paper›PMID 40690025›Full record

ArticleCell and tissue banking2025

Effect of the pulp harvesting method on the viability of human dental pulp stem cells and their odontogenic differentiation potential.

Justine De Visscher, Lore Vermeir, Natasja Van den Vreken, Liesbeth Temmerman, Noëmi De Roo, Jolanda van Hengel, Guy De Pauw

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Article in Cell and tissue banking, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Justine De VisscherDepartment of Oral Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium. Justine.devisscher@ugent.be.
Lore VermeirMedical Cell Biology Research Group, Department of Human Structure and Repair, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Natasja Van den VrekenMedical Cell Biology Research Group, Department of Human Structure and Repair, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Liesbeth TemmermanDepartment of Oral Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Noëmi De RooDepartment of Oral Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Jolanda van HengelMedical Cell Biology Research Group, Department of Human Structure and Repair, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Guy De PauwDepartment of Oral Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000, Ghent, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this study was to compare two methods for harvesting human dental pulp stem cells (hDPSCs) and investigate their impact on the DPSC viability and differentiation capacity. Healthy premolar teeth were collected from children aged 9 - 17 years old undergoing orthodontic treatment with two or four premolar extractions. The included premolars were randomly allocated to the apical group (removal of the dental pulp through the apical foramen after tooth extraction) or the coronal group (isolating the pulp tissue through the crown prior to tooth extraction). A total of 148 healthy premolar teeth from 46 patients were extracted, including 74 pulp tissues for the apical group and 74 for the coronal group. Due to bacterial and fungal infections, 37 (18 apical and 19 coronal) DPSC lines were obtained. Flow cytometric analysis and MTT assays showed no statistical differences in cell viability between the two methods. Odontogenic differentiation, assessed through immunocytochemical staining and Alizarin Red staining, also showed no visual or statistical differences between the apical and coronal approaches. In conclusion, the biological characteristics of hDPSCs harvested through the crown were equal to apically prelevated hDPSCs.

Indexed as

Cell DifferentiationDental PulpOdontogenesisStem CellsAdolescentBicuspidCell SurvivalChildFemaleHumansMaleCoronalDental pulpDental pulp stem cellDifferentiationTissue engineeringViability

Identifiers

PMID40690025

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.