Evidence map›Paper›PMID 40689951›Full record

ArticleAnalytical and bioanalytical chemistry2025

Isoreticular metal-organic framework-3 as a carrier of quantum dots for fluorescent immunolabeling in ultrasensitive lateral flow immunoassay of interleukin-6.

Shirong Zhang, Yuxin Tan, Yilei Liu, Yafei Li, Jishun Li, Shenglan Zhang, Hongcheng Pan

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shirong ZhangGuangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
Yuxin TanGuangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
Yilei LiuGuangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
Yafei LiGuangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
Jishun LiCollege of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541,004, China.
Shenglan ZhangCollege of Mechanical and Control Engineering, Guilin University of Technology, Guilin, 541,004, China.
Hongcheng PanGuangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China. panhongcheng@glut.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantum dots (QDs) have emerged as highly sensitive fluorescent markers for lateral flow immunoassays (LFIAs) due to their excellent fluorescence brightness and photostability. The incorporation of multiple QDs onto a carrier can further enhance detection sensitivity. However, traditional carriers such as polystyrene, while commercially successful, involve complex preparation processes and rely on organic solvents, which can lead to fluorescence quenching and adversely affect detection performance. In this study, we selected isoreticular metal-organic framework-3 (IRMOF-3), characterized by its porous structure, high surface area, and abundant amine functional groups, as the carrier. We synthesized IRMOF-3@CdSe/ZnS composite nanoparticles with an average diameter of 378 ± 46 nm by coupling carboxyl-modified CdSe/ZnS QDs to the IRMOF-3 surface through an aqueous synthesis method. These nanocomposites efficiently loaded a high density of QDs without aggregation, thereby maintaining strong fluorescence intensity. Moreover, the abundant amino and carboxyl groups on their surfaces facilitate efficient antibody conjugation, and the nanoparticles retain excellent water dispersibility after labeling. Utilizing IRMOF-3@CdSe/ZnS composite nanoparticles as immunomarkers, we developed a novel fluorescent LFIA for the detection of interleukin-6 (IL-6), a biomarker associated with various inflammatory and autoimmune diseases. The method achieved a limit of detection for IL-6 as low as 8.6 pg/mL, with relative standard deviations ranging from 2.73% to 6.14% and spiking recoveries between 96.4% and 110.8%. Passing-Bablok regression analysis demonstrated good consistency between our method and commercially available immunochromatography assays. The IRMOF-3@CdSe/ZnS composite nanoparticles, as a novel fluorescent label, exhibit considerable potential for applications in biomedical detection.

Indexed as

Interleukin-6Metal-Organic FrameworksQuantum DotsCadmium CompoundsFluorescent DyesHumansImmunoassayLimit of DetectionSelenium CompoundsSulfidesZinc CompoundsCadmium Compoundscadmium selenideFluorescent DyesInterleukin-6Metal-Organic FrameworksSelenium CompoundsSulfidesZinc Compoundszinc sulfideFluorescence detectionInterleukin-6Isoreticular metal–organic framework-3Lateral flow immunoassaysQuantum dots

Identifiers

PMID40689951

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.