An osteoblast-like cell line derived from mice expressing FRET-based tension sensor reveals cellular tension increase during osteogenic differentiation.

Junfeng Wang, Jeonghyun Kim, Eijiro Maeda, Takeo Matsumoto

Abstract read

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Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Junfeng WangBiomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.

Jeonghyun KimBiomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.

Eijiro MaedaBiomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.

Takeo MatsumotoBiomechanics Laboratory, Department of Mechanical Systems Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mechanical stimuli significantly influence bone remodeling, although the detailed molecular mechanisms involving changes in intracellular tension during osteoblast differentiation remain unclear. The present study was performed to investigate the role of intracellular tension in osteogenic differentiation by utilizing a newly established osteoblast-like cell line. In this study, we established a novel osteoblast-like cell line derived from calvarial explants of transgenic mice ubiquitously expressing a Förster Resonance Energy Transfer (FRET)-based tension sensor, capable of real-time measurement of intracellular tension and mineralization. The established cell line FRET1-MC8 displayed superior proliferative ability compared to conventional MC3T3-E1 osteoblast-like cells, maintaining stable growth and mineralization capability through at least passage 65. Osteogenic medium (OM) significantly enhanced the expression of osteogenic differentiation markers

Indexed as

Bone remodelingDifferentiationFRETMineralizationOsteoblastTension sensor

Identifiers

PMID40688497

DOI10.1016/j.bbrep.2025.102131

PMCPMC12270729

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.