ArticleDrug design, development and therapy2025
Astragaloside IV Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells via Activating PI3K/AKT/eNOS/NO Signaling Pathway: In vitro and in vivo Study.
Article in Drug design, development and therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Formononetin attenuates glucocorticoid-induced osteoporosis via multiple osteoimmunological pathways: evidence from zebrafish and network pharmacology.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2026Article
- H19 Promotes Odontogenic Differentiation of Human Dental Pulp Cells via miR-103a-3p-Mediated PIK3R1/AKT and KLF4 Pathways.International dental journal · 2026Article
- Diallyl Disulfide Mitigates LPS-Induced Inhibition of Osteogenic Differentiation and Alleviates Inflammatory Bone Loss via PI3K/AKT Signaling Pathway.Drug design, development and therapy · 2026Article
- Perilla Seed Oil Alleviates High-Fat-Diet-Induced Hyperlipidemia by Regulating Fatty Acid Metabolism via the PI3K/Akt/NOS3 Pathway.Foods (Basel, Switzerland) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Periodontal ligament stem cells (PDLSCs) play a critical role in alveolar bone regeneration and orthodontics. Astragaloside IV (AS-IV) is the chief ingredient of Astragalus, which has been shown to promote osteogenesis. The study aimed to detect the impact of AS-IV on osteogenic differentiation of PDLSCs and to investigate the role of the PI3K/AKT/eNOS/NO pathway in this process. Methods: PDLSCs were isolated from clinically healthy premolars that were extracted for orthodontic purposes from patients aged 14-20 years. The isolated cells were then cultured in vitro and characterized by flow cytometry. After treating the cells with different doses of AS-IV, LY294002 (PI3K inhibitor), and L-NAME (eNOS inhibitor), alkaline phosphatase (ALP) staining, alizarin red staining, qRT-PCR, Western blotting, nitric oxide (NO) assay and immunofluorescence staining were utilized to ascertain the expression level of related factors and the validity of PI3K/AKT/eNOS/NO pathway. Divided sixteen male Wistar rats into the control and AS-IV groups, and the orthodontic tooth movement model was created for 14 days. Micro-computed tomography scan, hematoxylin and eosin staining and immunohistochemical staining were conducted to investigate relevant indicators. Results: PDLSCs expressed high levels of surface antigens CD44 and CD90 while negatively expressing CD34 and CD45. AS-IV at each experimental concentration did not inhibit the proliferation of hPDLSCs, and 20 μM AS-IV could significantly enhance ALP activity, mineral deposition, and ALP, runt-related transcription factor 2 (RUNX-2), collagen I (COL-1) expression. After adding inhibitors LY294002 and L-NAME, the effect of AS-IV was inhibited. In vivo, AS-IV increased bone volume/total volume (BV/TV), trabecular thickness (Tb. Th), and the expression of ALP, COL-1 and eNOS on the tension side in rats. Conclusion: AS-IV can promote the osteogenic differentiation of PDLSCs, and PI3K/AKT/eNOS/NO was involved. Meanwhile, AS-IV exhibits positive effects on tension-side osteogenesis during tooth movement in rats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.