ReviewFrontiers in microbiology2025
Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases.
Review in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Drivers of the paradigm shift in norovirus diagnostics: technological innovation, contextual demands, and collaborative synergy.Virology journal · 2026Review
- Review
- Rapid visual detection of Mycoplasma gallisepticum by combining recombinase-aided amplification with lateral-flow dipstick assay.Poultry science · 2026Article
- A Rapid, Field-Deployable Diagnostic Platform for Getah Virus Based on RT-RAA and CRISPR EsCas13d.Microbial biotechnology · 2026Article
- Rapid On-Site Detection ofPlants (Basel, Switzerland) · 2026Article
- Advancements and Emerging Trends in the Detection of Sweet Potato Diseases: A Comprehensive Review.ACS omega · 2026Review
- One-pot recombinase polymerase amplification and CRISPR/Cas12a assay for Cryptococcus neoformans.Applied microbiology and biotechnology · 2026Article
- Multiplex on-chip detection ofFrontiers in bioengineering and biotechnology · 2026Article
- Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene.PloS one · 2026Article
- Rapid and simultaneous detection ofFrontiers in cellular and infection microbiology · 2026Article
- A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis.Annals of hematology · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid detection of infectious diseases is critical for global public health prevention and control. However, the use of traditional molecular diagnostic methods, including PCR, has been limited because of their cumbersome procedures, complex equipment requirements, operation at different temperatures, and the level of expertise required for operation. Isothermal amplification technology (IAT) provides a rapid, sensitive, specific, simple and less costly method for diagnosing infectious diseases, which has led to revolutionary breakthroughs in molecular diagnostics. This paper summarizes recent progress in IAT technology, which focuses on the principles and applications of core technologies such as NASBA, LAMP, RPA, and RAA. In addition, the combination of IATs with the CRISPR/Cas system, which further revolutionizes nucleic acid detection technology, is explored in this review.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.