Evidence map›Paper›PMID 40687827›Full record

ArticleiScience2025

Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae.

Yuheng Cai, Greta M Leitz-Najarian, Reuben Rosen, Kendall A Hutson, Adele Moatti, Chen Li, Douglas C Fitzpatrick, Alon Greenbaum

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Yuheng CaiLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.
Greta M Leitz-NajarianDepartment of Otolaryngology/Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Reuben RosenLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.
Kendall A HutsonDepartment of Otolaryngology/Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Adele MoattiLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.
Chen LiLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.
Douglas C FitzpatrickDepartment of Otolaryngology/Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Alon GreenbaumLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA.

Funding

Virology Research Program (Program 4)P30CA016086 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Deborah F. Tate · 1985 to 2026
$201.5M
Andor Dragonfly 202 Spinning Disk Confocal Microscope for the University of North Carolina at Chapel HillS10OD030223 · OD · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ARIEL, PABLO · 2021 to 2021
$474k
NCI NIH HHS P30 CA016086NIH HHS S10 OD030223
6 · The paper itself

Abstract

Tissue clearing and light-sheet fluorescence microscopy were applied for 3D profiling of intact cochleae. However, the spiral ganglion neurons (SGNs) remain relatively understudied compared to hair cells and supporting cells, especially in large animal models. Here, we: (1) introduced collagenase treatment to the current protocol of tissue clearing to enhance uniform antibody staining of SGNs within the pig cochlea and (2) adopted a deep learning object detection model to locate and count SGNs in large 3D datasets via Spiner (Spiral ganglion neuron profiler). Using this approach, Type I SGNs in intact gerbil and pig cochleae were detected and counted in 3D, and Spiner counts were consistent with manual counts. We believe broad adaptation of the method will improve understanding of the SGN population and their role in hearing loss. Codes for a user-friendly web interface were provided for model running and fine-tuning, making it accessible to those without programming experience.

Indexed as

Cellular neuroscienceOptical imagingSensory neuroscience

Identifiers

PMID40687827
PMCPMC12274850

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.