ArticleFrontiers in veterinary science2025
Effects of hesperidin and ascorbic acid combination on boar sperm quality during cryopreservation.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Hesperidin or ascorbic acid is an effective antioxidant that can protect frozen-thawed sperm from oxidative damage. The aim of the present study was to investigate the cryoprotective effects of hesperidin (Hsd) and ascorbic acid (AsA) on boar sperm quality during cryopreservation. Sperm samples were collected once a week for 3 weeks. The ejaculated semen from eight boars in each of three replicates were pooled and split into seven groups with different doses of Hsd (0, 15, 45) μM and/or AsA (0, 100, 300) μM added to the freezing extender. The sperm motility, membrane integrity, acrosome integrity, mitochondrial membrane potentials, DNA integrity, reactive oxygen species (ROS), malondialdehyde (MDA) concentration, antioxidant enzyme activities, and protein expression were measured after freezing-thawing to evaluate the qualitative parameters of boar sperm. The results showed that the sperm motility, plasma membrane integrity, acrosomal integrity, DNA integrity, and mitochondrial membrane potential were significantly improved in the 15 μM Hsd + 100 μM AsA group compared with the control and other treatment groups (
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