ArticleRSC advances2025
Unravelling the influence of choline chloride-based deep eutectic solvents on lysozyme: a comparative study of fructose and formic acid donors.
Article in RSC advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Spray Dried Lysozyme Microspheres: Morphological Evolution and Enzymatic Activity Retention.Pharmaceutical research · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in medicine and pharmaceutics have led to an increasing need for efficient structure stabilization methods for therapeutic proteins. Lysozyme is an antimicrobial enzyme present in mucosal secretions and animal and plant tissues and merited by a wide range of applications. However, its inherent vulnerability to high temperatures and external stress limits these applications. To overcome these drawbacks, biocompatible solvents (deep eutectic solvents (DESs)) are used to stabilize the structure and activity of lysozyme. Consequently, a comprehensive assessment was undertaken to evaluate the effects of two DES solutions-choline chloride-fructose (ChCl/F) and choline chloride-formic acid (ChCl/FA)-on the conformation, thermal stability and enzymatic activity of lysozyme. This was done using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, steady-state fluorescence, dynamic light scattering (DLS), circular dichroism (CD) measurements, transmission electron microscopy (TEM) and activity assays, as a function of DES concentration. ChCl/FA is shown to preserve the structural and thermal stability, and enhance the enzymatic activity of lysozyme, while ChCl/F was shown to destabilize lysozyme. Moreover, high concentrations of both DES solutions weaken the activity of lysozyme. Overall, DESs can be described as potential biocompatible, sustainable media for preservating the stability and activity of lysozyme.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.