Evidence map›Paper›PMID 40685642›Full record

ArticlePlant communications2025

The TaMYB44-TaMYB1 module regulates grain amylose biosynthesis and flour viscosity in wheat.

Sen Li, Guoyu Liu, Ziyan Wu, Dongxue Zhang, Chongyi Gan, Baoyun Li, Jie Liu, Zhongfu Ni, Qixin Sun, Rongqi Liang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sen LiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Guoyu LiuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Ziyan WuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Dongxue ZhangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Chongyi GanState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Baoyun LiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Jie LiuState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Zhongfu NiState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Qixin SunState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China.
Rongqi LiangState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), China Agricultural University, Beijing 100193, China. Electronic address: liangrq@cau.edu.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Starch content and composition in wheat endosperm are critical determinants of wheat yield and quality. Amylose content is a key parameter used to characterize starch viscosity in wheat flour, which influences pasting time, peak viscosity, and, consequently, the eating quality of flour-based foods. However, the transcriptional regulatory mechanisms governing amylose biosynthesis in wheat remain poorly understood. Here, yeast one-hybrid (Y1H) screening identified a MYB transcription factor, TaMYB44-4D, that binds to the TaWx-7A promoter. Electrophoretic mobility shift assays (EMSAs), Y1H assays, and transactivation assays demonstrated that TaMYB44-4D binds to the TaWx-7A promoter and represses its transcriptional activity. Knockout (KO) of TaMYB44-A/B/D simultaneously increases amylose content, total starch content, swelling power, and rapid viscosity analyzer parameters, whereas overexpression of TaMYB44-4D reduces these traits. Our results indicate that TaMYB44 functions as a transcriptional repressor of starch biosynthesis. We further confirmed that TaMYB44 interacts with TaMYB1; similar phenotypes were observed in tamyb44-KO and tamyb1-KO lines, as well as in TaMYB44-4D-overexpression (OE) and TaMYB1-6B-OE lines, suggesting that the two factors act as co-repressors. A single-nucleotide polymorphism (T/C) identified in the TaMYB44-D promoter among various wheat varieties is associated with differential TaMYB44 expression levels. Phenotype-genotype association analysis in a natural wheat population revealed that varieties with the CC haplotype exhibit higher amylose content than those with the TT haplotype. In conclusion, our findings provide important insights into the transcriptional regulation of amylose biosynthesis and offer a genetic framework for the improvement of wheat starch quality.

Indexed as

AmyloseFlourPlant ProteinsTranscription FactorsTriticumEdible GrainGene Expression Regulation, PlantPromoter Regions, GeneticStarchViscosityAmylosePlant ProteinsStarchTranscription Factorsamylose contenthaplotypeTaMYB1TaMYB44wheat

Identifiers

PMID40685642
PMCPMC12447431

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.