Evidence map›Paper›PMID 40685063›Full record

ArticleDevelopmental biology2025

IPPK-1 and IP6 contribute to ventral nerve cord assembly in C. elegans.

Nathaniel Noblett, Tony Roenspies, Chloe B Kirezi, Clover Stubbert, Stephane Flibotte, Pavak K Shah, Antonio Colavita

Abstract read

In one paragraph

Article in Developmental biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

VNC-Dist: A machine learning-based semi-automated pipeline for quantification of neuronal position in the C. elegans ventral nerve cord.PloS one · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nathaniel NoblettNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.

Tony RoenspiesNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.

Chloe B KireziNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.

Clover StubbertDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA.

Stephane FlibotteUBC/LSI Bioinformatics Facility, University of British Columbia, Vancouver, BC, Canada.

Pavak K ShahDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Institute for Quantitative and Computational Biosciences, University of California, Los Angeles, CA, USA.

Antonio ColavitaNeuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; University of Ottawa Brain and Mind Research Institute, Ottawa, ON, Canada. Electronic address: colavita@uottawa.ca.

Funding

Lineage-Specific Mechanisms of Cell Cycle Timing ControlR35GM151199 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Pavak Kirit Shah · 2023 to 2026

$1.5M

NIGMS NIH HHS R35 GM151199

6 · The paper itself

Abstract

Inositol phosphates (IPs) are essential for the development and function of the nervous system. Loss-of-function studies, which demonstrate the importance of specific IP isomers, show their critical role in proper neural tube formation. In this study, we show that inositol pentakisphosphate 2-kinase (IPPK-1), the kinase that phosphorylates IP5 to generate IP6, is involved in assembling the ventral nerve cord (VNC) in C. elegans. We show that mutations in ippk-1 lead to the mispositioning of motor neurons along the VNC of newly hatched larvae. These positioning defects reflect disruption of VNC assembly during embryogenesis, as VNC neuronal progenitors in ippk-1 embryos display a more compact organization after arising on the left and right sides of the embryo, delays in rosette-mediated convergent extension, and defects in cell intercalation. We further show that injection of exogenous IP6 into the gonads of ippk-1 mutants can rescue both embryonic and neuron positioning defects. Our findings indicate that IP isomers, particularly IP6, are important for ventral nerve cord formation in C. elegans. Along with their role in neural tube formation in vertebrates, these results suggests that IP isomers play an ancient role in central nerve cord development.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsInositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)AnimalsLarvaMotor NeuronsMutationNeural TubeCaenorhabditis elegans ProteinsInositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)Convergent extensionInositol phosphateIP6IPPKNerve cordSeptins

Identifiers

PMID40685063

DOI10.1016/j.ydbio.2025.07.007

PMCPMC13007942

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