ArticleCommunications biology2025
Construction and phenotypic classification of synthetic dual-pole Escherichia coli cells.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macromolecular complexes anchored at one cell pole have previously been utilized to achieve synthetic asymmetric cell division in Escherichia coli. In nature, however, robust asymmetric cell division often relies on gradients established between two opposing poles. Here, we present a second-generation synthetic genetic circuit that enables two distinct scaffolding proteins to localize at opposite poles in E. coli. By engineering a PodJ fragment as the second scaffold and integrating SpmX, a negative regulator of PodJ condensation, as a direct chimera with PopZ, we effectively confined PopZ and PodJ to opposite poles within E. coli. Additionally, we developed a neural network-based classification method to identify these synthetic dual-pole cells, further advancing the synthetic biology toolkit for programmable cell differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.