ArticleBulletin of experimental biology and medicine2025
Generation of Cell Models with Stable Expression of a Caspase-3 Activity Fluorescent Sensor.
Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Anastasis: Reality or Fiction?Bulletin of experimental biology and medicine · 2025Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lentiviral vector was created for inserting the commercial fluorogenic protease biosensor for caspase-3, ZipGFP-Casp3, into cell cultures. Three cultures of mesenchymal stem cells (MSCs) from the liver and placenta, dermal fibroblasts, and colorectal carcinoma clones of HT-29 and Caco2 cell lines with knockout of the main cancer stem cell marker CD133, along with control clones of these lines, were selected as the target cultures for studying apoptosis. All cultures were transduced with the ZipGFP-Casp3 sensor. The transduced cells were sorted, and in some derived cultures, cells undergoing spontaneous apoptosis and showing stable GFP fluorescence were noted. Additionally, experiments were conducted to study the sensitivity of transduced placental mesenchymal stem cells, as well as clones of the HT-29 line with CD133 knockout and its control clone, to classical apoptosis inducers staurosporine and TRAIL. The obtained results confirm that the derived cell cultures are good models for studying apoptosis in MSC and in the cancer stem cell population.
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