ReviewCurrent opinion in HIV and AIDS2025
A daisy chain of inferences: the role of single-cell and single-genome proviral sequencing in characterizing HIV-1 reservoirs.
Review in Current opinion in HIV and AIDS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Cytotoxic CD4 T Cells: Dual Agents in HIV-1 Pathogenesis and Persistence.Current HIV/AIDS reports · 2026Review
- Within-Subtype HIV-1 Polymorphisms and Their Impacts on Intact Proviral DNA Assay (IPDA) for Viral Reservoir Quantification.Viruses · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
purpose of reviewUnderstanding and targeting the HIV reservoir requires navigating a hierarchy of inferential assays. Quantitative viral outgrowth assays, FLIP-seq, and intact proviral DNA assays (IPDA) - though methodologically distinct - are all fundamentally single-cell technologies. Each relies on limiting dilution to isolate and interrogate individual proviral genomes derived from single infected cells, offering high-resolution proxies for the outcome of greatest interest: replication competence and the risk of viral rebound. Rather than providing direct measurements, these assays infer one another in a nested framework. This review highlights the importance of critically interpreting assay outputs within this chain of inference to guide cure-directed strategies and reservoir quantification. RECENT
findingsRecent studies emphasize the complexity and limitations of current assays measuring HIV-1 reservoirs. Key themes include reliance on bioinformatics definitions of genome-intactness to infer replication competence, alongside significant limitations due to viral diversity, PCR amplification length biases, and definitional inconsistencies. Modified assays like subtype-specific IPDA aim to address these issues. SUMMARY: Standardized, subtype-specific single-cell methodologies are crucial for accurate HIV reservoir characterization. Future research should integrate large-scale sequencing with replication competence validation, and should refine bioinformatics approaches to enhance predictive accuracy. Enhanced assay precision is essential to inform effective HIV cure strategies.
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