ArticleStem cell research & therapy2025
Enhanced neuromorphogenesis of neural stem cells via the optimization of physical stimulus-responsive signaling pathways.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Beyond Potency: Emerging Determinants and Optimization Strategies Enhancing Therapeutic Efficacy of Adult Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- 25+ Years of TRPV4: From Discovery to Translational Horizons.Research square · 2026Article
- Electrical Microenvironment Reconstruction and the Application of Biomaterials in Spinal Cord Injury.Journal of functional biomaterials · 2026Review
- Mechanisms and Applications of Conductive Biomaterials in Spinal Cord Injury Repair.Biomaterials research · 2026Review
- Mechanical cues as immunomodulators in neuroinflammation-driven spinal sensitization: analgesic mechanisms and therapeutic strategies.Frontiers in immunology · 2026Review
- Electrospinning for Mimicking Bioelectric Microenvironment in Tissue Regeneration.Research (Washington, D.C.) · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundNeural stem cells hold significant promise for developing in vitro nerve models due to their capacity to differentiate into diverse neural cell types. While traditional biochemical approaches often restrict differentiation to a single phenotype, limiting the ability to study critical neuron-glia interactions, physical stimuli have been explored due to their capacity to drive multi-phenotypic differentiation of neural stem cells. However, underlying molecular mechanisms mediating the physical stimulation-induced neural stem cell differentiation, with an emphasis on electrical stimulation and mechanical stimulation, remain inadequately explored, hindering the comprehensive optimization and application of physical stimulation for enhanced neuromorphogenesis.
methodsIn this study, we explored the signaling pathways driving mechano-electrical stimulation-induced multi-phenotypic differentiation of NSCs using a piezoelectric platform with signaling inhibitors. The signaling knowledge was then used to further enhance the neuromorphogenesis by employing signal activator/inhibitor in combination with mechano-electrical stimulation.
resultsWe discovered that electrical stimulation promotes the neuronal differentiation of NSCs via Wnt signaling through the TRPC1 channel, while mechanical stimulation activates the TRPV4-RhoA/ROCK axis, inducing astrocytic and oligodendrocytic differentiation via JAK/Stat3 and Shh/Gli1 pathways, respectively. Targeted modulation of these pathways under mechano-electrical stimulation further enhanced neuromorphogenesis, including improved neurite outgrowth, synaptic interactions, and myelin maturation.
conclusionsThis study systematically uncovered the signaling cascades that mediate the mechano-electrical stimulation-induced multi-phenotypic differentiation of neural stem cells towards neurons and glial cells, which were separately mediated by electrical stimulation and mechanical stimulation, respectively, through independent signaling cascades. The combination of physical stimulation with biochemical factors to modulate those signaling pathways further enhanced neuromorphogenesis, offering a reliable and robust strategy to develop fully functional neural stem cell-derived in vitro nerve models.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.