Evidence map›Paper›PMID 40682184›Full record

ArticleStem cell research & therapy2025

Ionizing radiation-mediated dendritic cell maturation exacerbates inflammatory response of bone marrow mesenchymal stem cells and impairs osteogenesis in radiation-induced jaw injury.

Mengting Zheng, Heng Chen, Zhonglong Liu, Zhouyang Wang, Ting Jiang, Yue He

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Mengting Zheng *Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, People's Republic of China.
Heng Chen *Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, People's Republic of China.
Zhonglong Liu *Department of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, People's Republic of China.
Zhouyang WangDepartment of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, People's Republic of China.
Ting JiangNational Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai, 200011, People's Republic of China.
Yue HeDepartment of Oral Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011, People's Republic of China. william5218@126.com.

Funding

Biomaterials and Regenerative Medicine Institute Cooperative Research Project, Shanghai Jiao Tong University School of Medicine 2022LHA09National Natural Science Foundation of China 82173451Shanghai Hospital Development Center SHDC2023CRT009Shanghai Municipal Health Commission 2022LJ001
6 · The paper itself

Abstract

backgroundRadiation-induced jaw injury is one of the most severe complications after radiotherapy for head and neck cancer, which can disrupt patients' health and quality of life. Although the direct target of inflammation and suppressed bone regeneration activity by ionizing radiation (IR) has been phenomenally observed, the underlying mechanisms and potential therapeutic targets remain blurred. Osteoimmunology emphasizes that dendritic cells (DCs) may contribute to bone diseases.

methodsIn this study, we assessed phenotypic and functional alterations of DCs in a radiation-induced jaw injury rat model through immunohistopathological staining. The effects of IR on bone marrow-derived dendritic cells (BMDCs) in vitro were further validated by flow cytometry, ELISA, mixed lymphocyte reaction (MLR) assay, and transwell. The cellular responses and differentiation of bone marrow mesenchymal stem cells (BMSCs) under BMDC-derived conditioned medium stimulation were evaluated through various cell staining, Quantitative real-time polymerase chain reaction (qRT-PCR), and western blotting. Flow cytometry, qRT-PCR, WB were employed to verify tolerogenic characteristics of Vitamin D3 (VitD3)-induced tolerogenic DCs (tolDCs). TolDCs were encapsulated in GelMA to develop an effective in vivo therapeutic approach for irradiated jaw defects.

resultsWe revealed that IR activated the mature-inflammatory phenotype and corresponding biological functions of DCs through the nuclear factor kappa-B (NF-κB) signaling pathway. Exposure to conditioned medium from irradiated BMDCs induced oxidative stress and inflammatory responses in BMSCs, inhibiting their proliferation, migration, and osteogenic potential while potentiating adipogenic capacity. Furthermore, tolDCs were proven to be resistant to radiation-induced activation. Local administration of tolDCs was effective in improving bone regeneration of irradiated jawbone defects.

conclusionsHyperactivation of DCs served as a potential pathogenic factor in radiation-induced jaw injury, exacerbating local inflammation and abrogating the biological functions of BMSCs. The local transplantation of tolDCs was a promising therapeutic strategy for osteogenesis in radiation-induced jaw injury.

Indexed as

Dendritic CellsJawMesenchymal Stem CellsOsteogenesisRadiation InjuriesAnimalsBone Marrow CellsCell DifferentiationInflammationMaleNF-kappa BRadiation, IonizingRatsRats, Sprague-DawleyNF-kappa BBone marrow mesenchymal stem cellsDendritic cellsLonizing radiationOsteoimmunologyOsteonecrosis of the jaw

Identifiers

PMID40682184
PMCPMC12275270

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.