ArticleVirology journal2025
Quantum dot immunochromatographic strip for rapid and sensitive detection of H5 subtype avian influenza virus.
Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed.
- A bispecific antibody targeting conserved hemagglutinin epitopes confers broad protection against H7 avian influenza and accelerates viral clearance via localized respiratory delivery.Emerging microbes & infections · 2026Article
- Smartphone-assisted upconversion nanoparticle assay for rapid multiplex detection of H5, H7, and H10 avian influenza viruses.Emerging microbes & infections · 2026Article
- A monoclonal antibody-based, time-resolved fluorescence microsphere immunochromatographic testing strip for rapid and sensitive detection of H7 avian influenza viruses.BMC veterinary research · 2026Article
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The H5 subtype avian influenza viruses (AIVs) pose a major threat to wild fowl and poultry. Additionally, they can overcome the species barrier, inducing human infection, which may become fatal. Thus, the H5 subtype AIVs remain a global public health burden, with a huge pandemic potential. Therefore, it is imperative to establish a rapid, sensitive, and specific method for detecting H5 subtype AIV infection for diagnostic and preventive purposes. The quantum dot fluorescent microsphere-based immunochromatographic strip (QDFM-ICS) is being widely used for detecting various viruses. We here developed a pair of monoclonal antibodies (2F2 and 2B7) by immunizing mice with the A/duck/Zhejiang/6D2/2013(H5N6) virus and covalently linking them with quantum dots to generate a QDFM-ICS for detecting H5 subtype AIVs. The QDFM-ICS showed a limit of detection of 0.0625 hemagglutination units (HAU) per 80 µL for live H5 subtype AIVs and 1.2 ng/mL purified H5N6 hemagglutination protein, respectively. In addition, it demonstrated good reproducibility with a coefficient of variation of < 10%, showing a high degree of repeatability. The strips exhibited a full percentage of specificity. Notably, their sensitivity and specificity remained unaffected even when stored for 3 months at room temperature or 4 °C. Furthermore, the application in practical testing on field samples demonstrated a strong correlation between QDFM-ICS and real-time PCR. The QDFM-ICS can be used in less time, with a simpler operation, and lesser expenditure. Thus, QDFM-ICS is a practical and promising technique for detecting H5 subtype AIVs, especially in point-of-care testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.