ArticleScientific reports2025
Conservation of Verbascum sinaiticum Benth using innovative tissue culture technique and DNA barcoding.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Egypt has diverse medicinal plants, many of which are endangered because of environmental and human pressures. Verbascum sinaiticum Benth (V. sinaiticum) is a medicinal plant from the Scrophulariaceae family and a locally rare species endemic to Sinai. This study introduces an innovative in vitro regeneration protocol for V. sinaiticum, developed for the first time using optimized tissue culture techniques. Additionally, the incorporation of molecular identification by DNA barcoding and biochemical profiling (via HPLC and SDS-PAGE) presents a complete strategy to conservation and genetic documentation, to support future biotechnological applications. An efficient in vitro regeneration system for V. sinaiticum was established using tissue culture techniques. The study investigated the effects of three different shoot induction media on growth, The first medium was BA medium, a combination of 0.5 mg L
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