Evidence map›Paper›PMID 40679658›Full record

ArticleParasitology research2025

Discovery of new chromosomal markers through repeatome analysis of Caryophyllaeus laticeps (Caryophyllidea).

Anna Marková, Martina Orosová

Abstract read
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Article in Parasitology research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Anna MarkováInstitute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia.ORCID http://orcid.org/0000-0002-0824-2026
Martina OrosováInstitute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia. orosm@saske.sk.ORCID http://orcid.org/0000-0001-8934-9126

Funding

Vedecká Grantová Agentúra MŠVVaŠ SR a SAV VEGA 2/0093/23
6 · The paper itself

Abstract

Genomic repetitive elements are essential structural and functional components of most eukaryotes and have attracted the attention of researchers in recent years. Despite the well-documented features of the karyotype of the tapeworm species Caryophyllaeus laticeps, knowledge about the content and distribution of repetitive elements on its chromosomes is limited. To gain a more comprehensive understanding of the composition of the repetitive DNA, we used a combination of bioinformatic, cytogenetic and molecular techniques to characterise the repetitive DNA content of this species. RepeatExplorer2 analysis revealed that transposable elements are predominantly present in the C. laticeps genome. The most suitable repeat families were selected for fluorescence mapping on the chromosomes, and most of them showed a scattered hybridisation pattern. In this study, we identified three new chromosomal markers: small nuclear U1 DNA (snDNA), the 5S ribosomal DNA (5S rDNA) and a satellite DNA named ClatSat14-167, each of which hybridises to a single locus at different chromosomal locations, allowing accurate identification of three pairs of chromosomes. These markers could be very valuable tools for future evolutionary studies and greatly enrich the limited cytogenetic data for tapeworms. In addition, an increased frequency of chromosomal aberrations was detected, reaffirming previous findings on the possible adverse effects of environmental pollution on the chromosomes of the species studied.

Indexed as

CestodaChromosomesRepetitive Sequences, Nucleic AcidAnimalsComputational BiologyDNA, HelminthDNA, RibosomalDNA, SatelliteGenetic MarkersIn Situ Hybridization, FluorescenceRNA, Ribosomal, 5SDNA, HelminthDNA, RibosomalDNA, SatelliteGenetic MarkersRNA, Ribosomal, 5SChromosomal aberrationsFluorescence in situ hybridizationRepeatExplorer2rRNAU1 snDNA

Identifiers

PMID40679658
PMCPMC12274263

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.