ArticleAnalytical methods : advancing methods and applications2025
Isolation and detection of target cells in blood
Article in Analytical methods : advancing methods and applications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The identification and quantification of cells in blood can serve to inform disease diagnosis and prognosis for patients. However, sample complexity presents a challenge for achieving sensitive and specific detection methods. Furthermore, there is a need to alleviate current standard clinical protocols from operator burden, limit the required sample volumes, and to reduce analysis timescales, all while maintaining sensitivity. This study presents a novel atomic emission cytometry assay for the detection of cells in blood with high specificity and sensitivity (LOD = 84 cells per μL), requiring only 500 μL sample volume and 1 hour of combined processing and analysis time, exhibiting the potential for broad applications in disease diagnosis. Metal nanoparticles equipped with antibodies serve as a targeted cell labeling platform, as well as an integrated method for immunomagnetic separation and subsequent quantification
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