The quantitative detection method employing a combination of high-affinity antibodies targeting PCT.

Xiaoxia Cheng, Lichen Zha, Jiao Yang, Yinyin Qin, Ruhong Yan, Yuzhu Ma, Changsong Zhang, Hongran Fu

Abstract read

In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Xiaoxia ChengDepartment of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, Jiangsu, China.

Lichen ZhaDepartment of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, Jiangsu, China.

Jiao YangSuzhou Research Center of Medical School, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, China.

Yinyin QinSuzhou Research Center of Medical School, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, China.

Ruhong YanDepartment of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, Jiangsu, China.

Yuzhu MaDepartment of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, Jiangsu, China.

Changsong ZhangDepartment of Clinical Laboratory, Suzhou Hospital, Affiliated Hospital of Medical School, Nanjing University, Suzhou, Jiangsu, China.

Hongran FuDepartment of Neurology, The Second People's Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and aims: Procalcitonin (PCT) is a widely recognized inflammation marker utilized in various clinical testing contexts and is subject to ongoing refinements, thereby imposing greater demands on core antibodies. However, the published literature lacks a comprehensive description of them. Material and methods: In this study, we initially expressed the full-length PCT protein in eukaryotic systems, followed by conventional antibody engineering techniques for Results: A total of 83 positive clones were generated, among which 15 high-affinity IgG Conclusion: This study presents a novel approach to enhancing the efficiency of antibody screening across a diverse array of combinations. Furthermore, the method established herein holds significant potential for clinical application in detecting PCT protein using FM-ICS.

Indexed as

High-affinity antibodyImmunofluorescence lateral flow assayProcalcitonin detection

Identifiers

PMID40678808

DOI10.1016/j.bbrep.2025.102091

PMCPMC12270020

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.