Evidence map›Paper›PMID 40678541›Full record

ArticleiScience2025

Sequencing airborne DNA to monitor crop pathogens and pests.

Amanda Mikko, Jose Antonio Villegas, Daniel Svensson, Edvin Karlsson, Per-Anders Esseen, Benedicte Riber Albrectsen, Ola Lundin, Mats Forsman, Anna Berlin, Per Stenberg

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Next-generation molecular tools in veterinary parasitology: advances, challenges, and perspectives in the diagnosis of emerging parasites.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Amanda MikkoUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden.
Jose Antonio VillegasDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden.
Daniel SvenssonDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden.
Edvin KarlssonDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden.
Per-Anders EsseenDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden.
Benedicte Riber AlbrectsenUmeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden.
Ola LundinDepartment of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Mats ForsmanCBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
Anna BerlinDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Per StenbergDepartment of Ecology and Environmental Sciences, Umeå University, Umeå, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Crop pests and diseases increasingly challenge the global food system. To prepare for and detect outbreaks, surveillance plays an important role. Traditional monitoring methods are often organism-specific, making large-scale monitoring of crop pathogens and pests impractical. We here investigate the potential for using shotgun sequencing of airborne eDNA for large-scale surveillance of crop pathogens and pests. We show that it is possible to detect DNA from all types of organisms in air, and that DNA can be classified down to species level. However, the accuracy of the identification is highly dependent on the quality of reference genomes of both the pathogens or pests, and their close relatives present in the region. Finally, we find that observed degree of crop damages correlate with amount of DNA from crop pathogens and pests in air, showing the promise of this approach for surveillance of all types of crop pathogens and pests.

Indexed as

Agricultural scienceEnvironmental biotechnologyEnvironmental monitoringGenomicsOmics

Identifiers

PMID40678541
PMCPMC12269458

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.