Evidence map›Paper›PMID 40676966›Full record

ReviewJournal of experimental botany2025

Plants in the expansion era: recent advances in expansion microscopy in plant systems.

Kevin L Cox, Kirk J Czymmek

Abstract readReview
In one paragraph

Review in Journal of experimental botany, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Kevin L CoxPlant and Microbial Biosciences Program, Division of Biology and Biomedical Sciences, Washington University in Saint Louis, St. Louis, MO 63130, USA.ORCID 0000-0003-2524-8988
Kirk J CzymmekAdvanced Bioimaging Laboratory, Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.ORCID 0000-0002-5471-7395

Funding

Howard Hughes Medical Institute 2130365
6 · The paper itself

Abstract

Expansion microscopy is an imaging method that overcomes the resolution barriers of traditional diffraction-limited light microscopes via isotropic physical expansion of biological cells, tissues, and organisms. This technique enhances the ability to visualize proteins, nucleic acids, and other biomolecules at nanoscale resolutions. While this approach has predominantly been applied to biomedical applications, workflows for plant systems have emerged. Here we review the development of expansion microscopy methods for the plant kingdom. These advances range from isolated organelles, single cells, and unicellular organisms to whole organs, embryos, and reproductive tissues. We highlight the unique advantages and findings from individual techniques and provide a perspective of potential directions that expansion microscopy can be applied to answer important plant research questions.

Indexed as

MicroscopyPlantsPlant CellsEmerging technologiesexpansion microscopyimagingsuper-resolution microscopy

Identifiers

PMID40676966
PMCPMC12707057

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.