Evidence map›Paper›PMID 40676631›Full record

ArticleJournal of ovarian research2025

circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression.

Xinxin Qin, Jinbi Zhang, Chao Yin, Fan Li, Wenjie Li, Xiaolong Cheng, Xing Du, Qifa Li, Zengxiang Pan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xinxin QinCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Jinbi ZhangCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Chao YinCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Fan LiCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, China.
Wenjie LiCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Xiaolong ChengCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Xing DuCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Qifa LiCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China.
Zengxiang PanCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing, China. owwa@njau.edu.cn.

Funding

National Natural Science Foundation of China 31672421
6 · The paper itself

Abstract

backgroundFollicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs).

methodsThe circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA.

resultsIn this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs).

conclusionsThe study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia.

Indexed as

ApoptosisGranulosa CellsMicroRNAsRNA, CircularAnimalsFemaleFollicular AtresiaSwineUp-RegulationMicroRNAsRNA, CircularcircRNAFollicular atresiaGranulosa cell apoptosismiRNATMX4

Identifiers

PMID40676631
PMCPMC12273050

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.