Evidence map›Paper›PMID 40676481›Full record

ArticleCell biochemistry and biophysics2025

In Silico and In Vitro Analysis of Bioactive Compounds from Ficus benghalensis as a Novel Approach to Targeting Brain Abscess Pathogen.

Ihtesham Arshad, Maryum Zainab, Ayesha Farooq, Saira Zulfiqar, Anna Ali, Rubina Bibi, Shaista Shafiq, Imran Zafar, Muhammad Mazhar Ayaz, Yahya A Almutawif and 1 more

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Article in Cell biochemistry and biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ihtesham ArshadSchool of Bioengineering, Dalian University of Technology, Dalian, China.
Maryum ZainabDepartment of Biotechnology, Lahore College for Women University, Lahore, Pakistan.
Ayesha FarooqDepartment of Biotechnology, University of Okara, Okara, Pakistan.
Saira ZulfiqarAllied Health Professionals, Faculty of LifeSciences, Government College University, Lahore, Pakistan.
Anna AliDepartment of Biotechnology, University of Okara, Okara, Pakistan.
Rubina BibiInstitute of chemical sciences bahauddinzakaryia University Multan, Multan, Pakistan.
Shaista ShafiqDepartment of Biochemistry and Biotechnology, Faculty of sciences, The University of Faisalabad(TUF), Faisalabad, Punjab, Pakistan.
Imran ZafarDepartment of Biochemistry and Biotechnology, Faculty of sciences, The University of Faisalabad(TUF), Faisalabad, Punjab, Pakistan.
Muhammad Mazhar AyazDepartment of Parasitology, Faculty of Veterinary Science, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan.
Yahya A AlmutawifDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
Najeeb Ullah KhanInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar, Pakistan. najeebkhan@aup.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The management of brain abscesses, particularly with Escherichia coli (E. coli) in immunocompromised patients, remains contentious. This study evaluates the bioactive potential of Ficus benghalensis extracts against brain abscess pathogens, including multidrug-resistant E. coli and Staphylococcus aureus (S. aureus), through phytochemical, pharmacological, and computational analyses. High yields (75-85%) were obtained from polar solvent extraction (acetone, methanol, ethyl acetate). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 30 bioactive compounds, including myricetin, naringenin-7-O-rutinoside, and harpagoside. Methanol extracts exhibited potent antimicrobial activity with inhibition zones of 18.2 mm (E. coli) and 17.9 mm (S. aureus) and minimum inhibitory concentration (MIC) values of 210-250 µg/mL. Ex vivo assays on clinical isolates showed dose-dependent inhibition (MIC50 = 150 µg/mL). Molecular docking indicated quercetin (-7.5 kcal/mol) and kaempferol (-7.8 kcal/mol) targeting E. coli FimH and OmpA, while lupeol (-9.1 kcal/mol) and ellagic acid (-8.7 kcal/mol) targeted S. aureus PBP2a and Hla. Pharmacokinetic analysis revealed quercetin and gallic acid as lead candidates with 100% gastrointestinal (GI) absorption and bioavailability (0.55-0.56), but limited blood-brain barrier (BBB) permeability (brain score: 0.24). Three-dimensional quantitative structure-activity relationship (3D-QSAR) comparative molecular field analysis (CoMFA) models (R

Indexed as

Anti-Bacterial AgentsBrain AbscessFicusPlant ExtractsComputer SimulationEscherichia coliHumansMicrobial Sensitivity TestsMolecular Docking SimulationStaphylococcus aureusTandem Mass SpectrometryAnti-Bacterial AgentsPlant ExtractsAntimicrobial activityBrain AbscessE. coliFicus benghalensisMolecular dockingPhytochemical analysis

Identifiers

PMID40676481

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.