Evidence map›Paper›PMID 40676339›Full record

ArticleArchives of microbiology2025

Transcriptomic analysis reveals differential gene expression in spleen cells of Chinese soft-shelled turtle infected with Bacillus cereus BC12.

Chun Fang, Wenjie Hu, Xiaowei Fang, Xiongyan Liang, Jing Liu, Yuting Yang

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chun FangCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China.
Wenjie HuCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China.
Xiaowei FangCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China.
Xiongyan LiangCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China.
Jing LiuCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China.
Yuting YangCollege of Animal Science and Technology, Yangtze University, No. 88, Jingmi Road, Jingzhou, 434025, China. 2023710921@yangtzeu.edu.cn.

Funding

National Natural Science Foundation of China 31802208Science and Technology Research Project of Education Department of Hubei Province Q20221302
6 · The paper itself

Abstract

Bacillus cereus infection has emerged as a leading cause of high mortality of Chinese soft-shelled turtle (Pelodiscus sinensis) in aquaculture. Elucidating the complex physiological processes and differentially expressed genes (DEGs) of P. sinensis in response to bacterial pathogens will help us explore strategies to combat bacterial infection. In this study, Illumina-based RNA-Seq was used to analyze the significantly active DEGs and pathways in the spleen of P. sinensis challenged by B. cereus BC12. A total of 973 DEGs were identified. These DEGs were significantly enriched in 5,421 Gene Ontology (GO) terms and 147 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways through enrichment analysis. The significantly active pathways included NOD-like receptor signaling pathway (pss04621), cytosolic DNA-sensing pathway (pss04623), cell cycle (pss04110) and spliceosome (pss03040). At 48 h post-infection (hpi), the NF-κB signaling pathway was downregulated, and cell cycle-related gene regulation was disrupted. Additionally, the expression of key spliceosome genes was downregulated. In summary, our results demonstrated the active relevant DEGs and pathways in the splenic cells of P. sinensis at 48 hpi with B. cereus BC12. These results offer valuable insights into the investigation of the molecular mechanisms underlying the resistance of P. sinensis to microbial infections.

Indexed as

Bacillus cereusGram-Positive Bacterial InfectionsSpleenTranscriptomeTurtlesAnimalsGene Expression ProfilingSignal TransductionBacillus cereusCellular processesChinese soft-shelled turtleGenetic information processingImmune systemTranscriptomic analysis

Identifiers

PMID40676339

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.