Evidence map›Paper›PMID 40676266›Full record

ArticleProtoplasma2026

Development of tobacco chloroplast vector with novel insertion sites for dengue virus (DENV) antigens.

Hailah M Almohaimeed, Rasha Assiri, Waheeb S Aggad, Meshal M ALsharafa, Sara F Faloudah, Ahmed M Abdulfattah, Fayez Alsulaimani, Ahmed M Basri

Abstract read
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Article in Protoplasma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hailah M AlmohaimeedDepartment of Basic Science, College of Medicine, Princess Nourah bint Abdulrahman University, P. O. Box 84428, 11671, Riyadh, Saudi Arabia. hmalmohaimeed@pnu.edu.sa.
Rasha AssiriDepartment of Basic Medical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P. O. Box 11671, Riyadh, Saudi Arabia.
Waheeb S AggadDivision of Anatomy, Department of Basic Medical Sciences, College of Medicine, University of Jeddah, 23890, Jeddah, Saudi Arabia.
Meshal M ALsharafaTissue Biobank Section, Natural and Health Science Research Center, Princess Nourah bint Abdulrahman University, P. O. Box 11671, Riyadh, Saudi Arabia.
Sara F FaloudahTissue Biobank Section, Natural and Health Science Research Center, Princess Nourah bint Abdulrahman University, P. O. Box 11671, Riyadh, Saudi Arabia.
Ahmed M AbdulfattahDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Fayez AlsulaimaniDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
Ahmed M BasriDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.

Funding

This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. "(RGP-1444-0061)". "(RGP-1444-0061)".
6 · The paper itself

Abstract

Dengue virus (DENV) is a substantial global health threat with no specific antiviral treatment available. The development of effective vaccines is crucial to combat DENV infections. Plant-based expression systems, such as tobacco chloroplasts, have emerged as promising platforms for the production of DENV antigens. In this research article, we describe the construction of a tobacco chloroplast vector with novel insertion sites for DENV-1 protein III. Our present study successfully demonstrated the production of the envelope protein structural domain III (EDIII) dengue virus antigen in tobacco chloroplasts. We obtained transformed tobacco lines that show the expression of EDIII dengue antigen and were confirmed through Southern blotting analysis. Furthermore, the expression of EDIII antigen was further confirmed via immunoblotting and quantified. These findings suggest that tobacco chloroplast engineering holds promise as a method for future manufacture of an oral dengue boost vaccine that is safe, affordable, and effective, contributing to the global management and control of this particular dengue infection.

Indexed as

Antigens, ViralChloroplastsDengue VirusGenetic VectorsNicotianaPlants, Genetically ModifiedAntigens, ViralDENVEDIIITobacco chloroplastVaccine

Identifiers

PMID40676266

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.