Evidence map›Paper›PMID 40675336›Full record

Trial reportThe Journal of nutrition2025

Acute Effects of Oral Microbial Protease Co-ingestion with Whey Protein on Postprandial Plasma Amino Acid Concentrations, Appetite, and Satiety in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled, Crossover Clinical Trial.

Yijia Huang, Zachary W Bell, Alyasamin Alhamwi, Benjamin Sauvageau, Divine Malenda, Silar Gardy, Thalia Krauth-Ibarz, Sarkis J Hannaian, José A Correa, Ari Gritsas and 5 more

Registry-linked trialAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05957185 (Effects of Oral Microbial Protease Supplementation on Postprandial Plasma Amino Acid Concentrations and Appetite in Healthy Adults), which is not on this map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05957185 nacompletednot on this map

Effects of Oral Microbial Protease Supplementation on Postprandial Plasma Amino Acid Concentrations and Appetite in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled, Crossover Clinical Trial

TypeinterventionalSponsorBIO-CAT, Inc.Ran2023 to 2024Enrolled24ConditionsDigestive Health, Gastrointestinal Health, Appetitive BehaviorArmsOPTIZIOME® P³ HYDROLYZER® - WHEY, Placebo
3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yijia HuangDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Zachary W BellDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada; Department of Clinical Health and Applied Sciences, University of Houston-Clear Lake, Houston, TX, United States.
Alyasamin AlhamwiDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Benjamin SauvageauDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Divine MalendaDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Silar GardyDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Thalia Krauth-IbarzDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada.
Sarkis J HannaianDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada; Research Institute of the McGill University Health Centre, Montréal, Quebec, Canada.
José A CorreaDepartment of Mathematics and Statistics, McGill University, Montréal, Quebec, Canada.
Ari GritsasResearch Institute of the McGill University Health Centre, Montréal, Quebec, Canada.
Sean M GarveyDepartment of Research and Development, BIO-CAT, Inc, Troy, VA, United States.
Kelly M TinkerDepartment of Research and Development, BIO-CAT, Inc, Troy, VA, United States.
Sidney Abou SawanIovate Health Sciences International, Oakville, Ontario, Canada.
José A MoraisResearch Institute of the McGill University Health Centre, Montréal, Quebec, Canada; Division of Geriatric Medicine, McGill University, Montréal, Quebec, Canada.
Tyler A Churchward-VenneDepartment of Kinesiology and Physical Education, McGill University, Montréal, Quebec, Canada; Research Institute of the McGill University Health Centre, Montréal, Quebec, Canada; Division of Geriatric Medicine, McGill University, Montréal, Quebec, Canada. Electronic address: tyler.churchward-venne@mcgill.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundProteases are enzymes that breakdown proteins into peptides and amino acids. When co-ingested with dietary protein, proteases may enhance digestion, increase postprandial plasma amino acid concentration, and affect gut hormones, appetite, and/or satiety.

objectivesThe aim of this study was to assess the effects of a mixture of 3 microbial protease preparations (P3) on postprandial plasma amino acid concentration when co-ingested with whey protein concentrate (WPC) in healthy young adults.

methodsP3 was first tested in vitro for proteolytic effects in a static simulation of orogastric digestion. In a subsequent randomized, double-blinded, placebo-controlled crossover study, 12 males and 12 females [body mass index (BMI)-mean: 23.6 (SD: 2.9); age-mean: 25 y (SD: 3 y)] consumed WPC (25 g protein) containing P3 or placebo (maltodextrin). Plasma amino acid, glucose, insulin, and appetite regulatory hormone concentrations were assessed at baseline and throughout a 240-min postprandial period. Perceived appetite sensations were assessed by visual analog scale questionnaires. An ad libitum meal was administered following each treatment to determine energy intake.

resultsP3 demonstrated proteolytic activity at 50,000 hemoglobin units on tyrosine basis per 31.9 g serving of WPC in vitro. Adjusted geometric mean postprandial plasma 60-min incremental area under the curve was 14% greater for essential amino acids (treatment: P = 0.025) and 15% greater for branched-chain amino acids (treatment: P = 0.021) with P3 than placebo, with no differences for total amino acids or leucine (all P > 0.05). Adjusted geometric mean postprandial plasma ghrelin was 12% lower (treatment: P < 0.001), whereas adjusted mean visual analog scale-derived fullness (treatment: P = 0.025) and satiation (interaction: t = 30-150 min; all P < 0.05) were greater with P3 than placebo. Adjusted mean postprandial plasma glucose, insulin, and ad libitum meal energy intake were not different between treatments (all P > 0.05).

conclusionsCo-ingestion of WPC with P3 can enhance early postprandial plasma aminoacidemia and alter select indices of appetite and satiety in young adults. This trial was prospectively registered at clinicaltrials.gov as NCT05957185.

Indexed as

Amino AcidsAppetitePeptide HydrolasesPostprandial PeriodSatiationWhey ProteinsAdultBlood GlucoseCross-Over StudiesDouble-Blind MethodFemaleHumansInsulinMaleYoung AdultAmino AcidsBlood GlucoseInsulinPeptide HydrolasesWhey Proteinsamino acidsappetitedietary proteindigestiondigestive enzymesmicrobial proteasesatietywhey protein

Identifiers

PMID40675336
PMCPMC12799412

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.