ReviewInternational journal of biological macromolecules2025
A review of advanced strategies for molecular weight determination of insoluble polysaccharides: Developments and future trends.
Review in International journal of biological macromolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Identifying Key Bioactive Components in Postbiotic Preparations: From Candidate Discovery to Functional Validation.Foods (Basel, Switzerland) · 2026Review
- Natural Polysaccharide-Mediated Nano-Delivery Systems for Osteoporosis Therapy From a Gut-Bone Axis Regulatory Perspective.Advanced healthcare materials · 2026Review
- Fractionation and Functional Characterization ofFoods (Basel, Switzerland) · 2026Article
- The non-scaffold effects of natural polysaccharides in promoting organ regeneration: a review.Journal of biological engineering · 2026Review
- Review
- Endophytic bacteria and fungi as a source of polysaccharides: production and characterization techniques.Frontiers in microbiology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Molecular weight is a critical factor in understanding the chemical and physical properties of polysaccharides. For soluble polysaccharides, many established and reliable methods are available. However, determining the molecular weight of insoluble polysaccharides presents significant challenges. Traditional methods like gel-permeation chromatography/size-exclusion chromatography (GPC/SEC), nuclear magnetic resonance (NMR), and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are typically unsuitable for direct application in analyzing insoluble polysaccharides. This review explores the innovative strategies designed to address these obstacles, categorizing them into two primary groups: intact and non-intact methodologies. Intact approaches include techniques like solid-state NMR, Fourier Transform Near-Infrared (FT-NIR) spectroscopy and fluorescence lifetime measurements which enable direct molecular weight determination without additional processing. In contrast, non-intact methods, such as derivatization processes, the use of dipolar aprotic solvents, and ionic liquids, involve dissolving the polysaccharides in specialized solvents before analysis with conventional instruments like NMR, MALDI, or SEC. This review discusses the advantages and limitations of each strategy, highlights the emerging role of molecular modeling in characterizing complex polysaccharide structures, and outlines future trends and advanced techniques that promise to enhance analytical accuracy. It also provides a comparative analysis to guide researchers in selecting the most appropriate method for their specific polysaccharide study needs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.